Antibacterial resistance: A comparative study between clinical and environmental isolates based on whole cell protein profiling by SDS-PAGE

Abstract

This study of comparison was conducted to examine the existence of antibiotic resistant and sensitive bacteria in various environmental samples of Bangladesh with specific clinical strain obtained from ICDDR,B. From the month April 2014 to September 2014, these environmental samples were isolated from different locations of Dhaka city to see the inclination of the environmental isolates to develop antibiotic resistance at different times of a year. The bacterial isolates were identified on the basis of standard cultural, morphological and biochemical attributes. Antibiogram was done to determine the antibiotic susceptibility of the isolates in the way that follows CLSI (Clinical & Laboratory Standards Institute) guideline. The after effect of this study showed all the clinical isolates were sensitive to these antibiotics whereas all the environmental isolates have become resistant to the tested antibiotics, and some of them have emerged to be multi-resistant to these therapeutic agents. The study serves to anticipate the future emergence and aide the advancement of strategies and methods to counteract this resistance. In this way intermittent and far reaching survey of antibiotic resistance in the natural microbes is needed.