Biomedical waste analysis

Abstract

The potential for biomedical waste to transmit infectious illnesses and include microorganisms resistant to antibiotics makes it a severe problem. The purpose of this study was to determine the microorganisms like Pseudomonas, Salmonella, Shigella, and E.coli were in biomedical waste The study was conducted from June 2023 to June 2024. A total of 39 samples were gathered from five different hospitals in Dhaka. From the total samples, 87 isolates of our targeted organism were identified. To identify presumed bacterial isolates, biochemical assays such as Triple Sugar Iron test, Oxidase test, Citrate Utilization test, Gram staining, and Catalase test were employed in combination with selective media. Furthermore, molecular identification of the 87 bacterial isolates was held using PCR. In addition to this, an antibiotic susceptibility test was carried out to find the resistance of these isolates to different antibiotics. The results from the tests showed a surprising number of confirmed targeted species. To begin with, approximately, 65% of the isolates were multi-drug resistant (MDR), with Pseudomonas species exhibiting the greatest level of resistance. In addition, it was found that Escherichia coli was present in 28% of the samples, and 65% of the isolates showed signs of multi-drug resistance (MDR). Also in about 22% of the samples, Shigella was found and every isolate had multiple drug resistance. Ultimately, 20% of the samples had Salmonella detected in them, and half of the isolates had multi-drug resistance. Overall, the results show a concerning frequency of infections that are resistant to several drugs in biomedical waste, underscoring the pressing need for improved infection control and waste management procedures to protect the public's health and stop the spread of these harmful organisms.