Characterization of newly identified bacterial biofilm degrading bacteriophage obtained from Dhaka city lake water samples

Abstract

Recently acquired bacteriophages from water samples in Dhaka city which degraded the bacterial biofilms were characterized in this investigation. Bacteriophages unique to E. coli strain 9A(ⲫ9A) and Vibrio cholerae strain 13B(ⲫ13B) were identified. Throughout the process, strict laboratory protocols were followed, and the characterization was based on three variables: temperature, pH, and salinity. In the first step of the procedure, single colonies of host bacteria were obtained by streak plating, which was followed by enrichment of the corresponding bacteriophages. The Phage counts were then determined using the Double Layer Assay (DLA). To begin DLA, single colonies in LB broth were first taken and cultured in a shaker. The bacteriophages underwent a series of dilutions before the phages and the bacteria were added in the molten soft agar. After being centrifuged, the soft agar was quickly placed onto a Luria Bertani Agar plate. After evenly distributing the materials, the plate was swirled and until the agar solidified and was then placed in an incubator for 16 hours. The following day, the plaques were counted, and the number of Plaque Forming Units per Milliliter (PFU/ml) were determined. An average phage count was discovered after the experiment was conducted three times for each component. Both ⲫ9A and ⲫ13B displayed unusual results in temperature and pH readings in addition to salinity.