Isolation and molecular characterization of e.coli bacteriophages from surface water of Bangladesh

Abstract

In our country Diarrheal epidemics occur seasonally. Two peaks of outbreaks agreeably coincide with dry season and monsoon rain. Several factors control the outbreaks to occur and collapse. Bacteriophages are one of them which have been reported to trigger the collapse of the outbreaks. The concentration of the Vibrio cholerae specific bacteriophages is inversely correlated with the concentration of Vibrio cholerae in the environment. Therefore bacteriophages probably play an essential role in controlling the epidemics to occur or collapse. It is still not clear what factors trigger the onset of Diarrheal outbreaks. This study was design to see the effect of E. coli bacteriophages on the epidemics of Diarrheal disease. Routine isolation, estimation and molecular characterization reveal the prevalence E. coli phage. We have tried to characterize the isolated phages by analyzing the DNA using the technique called restriction fragments length polymorphism (RFLP).