A study of microbial water quality check and isolation of Helicobacter Pylori from water samples taken from Dhaka city

Abstract

Water is indispensable for human survival. According to the World Health Organization, around two billion people in the world drink fecal contaminated water. This means there can be higher chances for the presence of the Helicobacter pylori in drinking water. H. pylori infection is the most common cause for gastric ulcer. In this study, water samples were collected in sterile autoclaved bottles from different areas of Dhaka City. Different media were used to detect fecal coliform, total coliform and differentiated colonies of H. pylori. The identification of H. pylori was carried out by biochemical tests, and PCR followed by gel electrophoresis. A 520 base pair of H. pylori DNA was found in the result of gel electrophoresis. From the result of this study, it is found that the presence of fecal and total coliform is at an alarming rate in the underprivileged areas (Korail slum and Bossila slum) than privileged areas. H. pylori has also been successfully isolated from the water samples taken from Korail slum.