Structural analysis of three pivotal SERK dependent PRR complexes of Arabidopsis thaliana

Abstract

Pattern Triggered Immunity (PTI) is distinguished with the activities of pattern recognition receptors (PRRs), which play an essential role in plant defense mechanism. During aggression of microbes, PRRs immediately bind with the PAMPs and recruit co-receptors to initiate the defense signal. Several plant PRRs have been discovered, very few of their functional parameters have been studied. In this study, the crystallographic structures of FLS2-flg22-BAK1 (PDB ID: 4MN8), HAESA-IDA-SERK1 (PDB ID: 5IYX) and PSKR-Phytosulphokine-SERK1 (PDB ID: 4Z64) complexes were simulated for 30ns. Simulated trajectories were analyzed for getting an overview of the immune response of PRRs towards PAMPs with the help of co-receptors. Moreover, MM/PBSA calculation revealed that interaction between FLS2-BAK1 and HAESA-SERK1 show similarity whereas PSKR interacts differently with SERK1. As PRRs play a major role in plant defense mechanism, it can be hypothesized that binding mechanism with PAMPs and co-receptors will help to understand the interaction pattern of PTI.