Investigation of In-vivoantidiarrhoeal and insecticidal activity of Trewianudijlora leaf extract

Abstract

Trewianudijlorais the plant that we choose for our thesis work. From the previous experiment it has been seen that leaf extracts of this pant contains tannins, cardiac glycosides, flavonoids etc and many more. These components shows different kind of phamacological action against various disease which is clinically proven. Keeping this in mind our current study was designed to find out Anti-dian·heal and Insecticidal activity from different solvent extracts (ETE-Ethanol, EAE-Ethyl-acetate, and CHE-Chloroform) ofleaves ofthis plant. Diarrhea is one of the alarming disease of Bangladesh.Dian·hea is defined by the World Health Organization(WHO) as having three or more loose or liquid stools per day, or as having more stools than is notmal for that person. Sometimes this secretion is so high that it may threaten people's life especially children. Our study was aimed to investigate of anti-diarrheal effect and insecticidal effect of Trewia nudiflora plant leaf extracts These chemical components are actually pharmacologically active against different diseases. As this plant contains tannins and flavonoids, so it can be assumed that it can be helpful to tighten intestinal motility. To tun the experiment we took ETE, EAE and CHE extract of the specified leaf. Two methods were followed to investigate antidiarrheal effect. They were: castor oil and magnesium sulphate induced diarrhea in the albino mice. In both cases we found that EAE extracts at 400mg/ml shows the best result(90.2%and 88.65%) comparing to the standard (Loperamide 5mg/kg) dtugs. So we can assume that EAE extracts of this leaf shows the maximum results which is approximate to the alopathy medicine. As it is natural so it will not possesses such side-effects though we increase the dose. Bangladesh is an agricultural country. Around 65% people's earning way is farming. Rice is the main crop that our frumers wish to grow in huge amounts. But, paddy sometimes attacked by the insects which in tum causes huge losses. Fatmers are using pesticides which are not eco-friendly as well as costly also. If we can replace these pesticides with some natural remedies it will be beneficial for both fatmers and environment. We followed Abbot's law to get the result and after plotting it in the graph it is clearly seen that EAE at highest dose gives the best result(83%).So, above experimental results indicate that bioactive compounds ru·e present in those three extracts of Trewia nudiflora leaves including antidiarrheal activity and insecticidal activity and could be accounted for phatmacological effects.