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dc.contributor.advisorIslam, Aparna
dc.contributor.advisorHoque, Ekramul
dc.contributor.authorRiva, Sahida Siraj
dc.date.accessioned2017-02-27T10:43:42Z
dc.date.available2017-02-27T10:43:42Z
dc.date.copyright2016
dc.date.issued2016-04
dc.identifier.otherID 13376002
dc.identifier.urihttp://hdl.handle.net/10361/7797
dc.descriptionA Dissertation Submitted in Partial Fulfillment of the Requirements’ for the Degree of Master of Science in Biotechnology 2016.en_US
dc.descriptionCataloged from PDF version of thesis report.
dc.descriptionIncludes bibliographical references (page 63-67).
dc.description.abstractThe effect of different concentrations of BA and IBA on in vitro plant regeneration was studied in both varieties of Dendrobium bensoniae and Dendrobium aphyllum. The composition of the culture medium using different hormone along or in combination affected the induction, regeneration and multiplication of both varieties of Dendrobium orchid. BA with 0.5, 1.0, 1.5 and 2.0 mg/l and IBA with 0.5, 1.0, 1.5 and 2.0 mg/l were used in both varieties. Combined hormonal concentrations were also used. Shoot regeneration using nodal explants was found to be best in 2.0 mg/l BA supplementation on MS medium, which gives better responses than all other combinations of BA and BA+IBA concentrations under study in both D. bensoniae and D. aphyllum. In hormonal combination, highest number of shoots and leaves were found using 1.0 mg/l BA and 1.5 mg/l IBA along with MS medium in both varieties. The highest number and maximum length of roots were noticed using 1.5 mg/l IBA in D. bensoniae. Similarly, highest number and maximum length of roots were found in concentration of 2.0 mg/l in case of D. aphyllum. In hormonal combination, 0.5 mg/l BA and 1.0 mg/l IBA with MS medium found to be most effective for root induction in both varieties of D. bensoniae and D. aphyllum. The well-rooted plantlets were successfully acclimatized, planted in pots and transferred to the shade house for establishment. So MS medium supplemented with 2.0 mg/l BA may be used for rapid shoot induction, regeneration and multiplication and 0.5 BA+1.0 IBA (mg/l) supplemented with MS medium may be used for rapid induction of root in D. bensoniae and D. aphyllum.en_US
dc.description.statementofresponsibilitySahida Siraj Riva
dc.format.extent67 pages
dc.language.isoenen_US
dc.publisherBRAC Universityen_US
dc.rightsBRAC University thesis are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission.
dc.subjectDendrobium bensoniaeen_US
dc.subjectDendrobium aphyllumen_US
dc.titleIn Vitro regeneration and rapid multiplication of two orchid varieties of Dendrobium bensoniae and Dendrobium aphyllumen_US
dc.typeThesisen_US
dc.contributor.departmentDepartment of Mathematics and Natural Sciences, BRAC University
dc.description.degreeM. Biotechnology


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