Thank you, a good research antioxidant, antimicrobial and toxicity studies of the different fractions of fruits of Terminalia belerica Roxb

Abstract

The crude methanolic extract of the fruits of Terminalia belerica Roxb. along with its various organic fractions were tested to evaluate the toxicity, antioxidant (in vitro and in vivo) and antibacterial activity. The different assay methods, including total antioxidant activity, scavenging free radical, authentic peroxynitrite and reducing power assessment were used to evaluate the antioxidant potential of the crude extract and its organic fractions. While antimicrobial activities were evaluated using agar disc diffusion method and measuring their MIC value. The EtOAc fraction, showed strong activity in all the model systems tested and in peroxynitrite model this fraction (IC50= 1.29±0.18 μg/ml) exerted four-fold stronger activity than standard penicillamine (IC50 value of 5.20±0.32 μg/ml). The reducing power of the extract was found to be concentration dependent. 50 The administration of the extract/fractions at a dose of 200 and 400 mg/kg body weight to the male Wistar rats increased the percentage inhibition of reduced glutathione, superoxide dismutase and catalase significantly. Whereas, lipid peroxidation level in hepatotoxic rats markedly decreased at a dose of 400 mg/kg body weight after 7 days. EtOAc fractions showed also the highest antibacterial effect against Staphylococcus aureus with zone of inhibition 30.00±0.25 mm. The Brine shrimp lethality bioassay was used to determine the toxicity of the extracts where dichloromethane (CH2 Cl2) fraction showed highest activity (LC50= 39.02±1.16 μg/ml). In conclusion, the study clearly indicated that the extract/fractions of Terminalia belerica Roxb possesses good antioxidant and antimicrobial activity along with moderate toxicity.