A comparative study of the antibiotic resistance pattern between the natural and clinical isolates based on whole cell protein profiling by SDS-PAGE

Abstract

The comparative study was executed to detect and examine the presence of antibiotic susceptible and antibiotic resistant bacteria in several environmental samples of Bangladesh. A specific clinical strain was obtained from ICDDR,B. Numerous environmental samples were collected from different locations of Dhaka city in order to verify the consistency of the environmental strains and their pattern of antibiotic resistance. All the standard biochemical and morphological tests were conducted to confirm the bacterial strains, after the isolates were cultured in a standard selective medium. Only, one single strain was chosen for the further study. Following CLSI (Clinical & Laboratory Standards Institute) guideline, the antibiotic sensitivity of the isolates was determined by carrying out antibiogram. The follow up of the entire study shows that the clinical strains were either resistant or had intermediate effects against most of the antibiotics, while the environmental strains were either sensitive or had intermediate effects against most of the antibiotics with an exception of one or two antibiotics against which these strains were resistant. The whole study provides an idea about the future emergence and the development of more counterproductive strategies to overcome this serious problem of multidrug resistance amongst the pathogenic organisms. The research work can be extended with the development of laboratory facilities, and in near future the characterization of the antibiotic resistant proteins of these organisms can be done.