Association of MTHFR rs1801133, PEMT rs4244593, CHKA rs7928739, VNTRs IL-1RN intron 2 and NOS3 intron 4 polymorphisms with unexplained intrauterine fetal death in Bangladeshi population
Abstract
This case-control study aimed to analyze interaction between increased risk of Intrauterine
Fetal Death in Bangladeshi pregnant women and Single Nucleotide Polymorphism (SNP) of
MTHFR (rs1801133), PEMT (rs4244593), CHKA (rs7928739), IL-1RN (intron 2 VNTR),
and NOS3 (intron 4 VNTR). This study comprised 62 cases of IUFD and 64 controls,
employed PCR/RFLP technique for MTHFR, PEMT, and CHKA gene, and only real-time
PCR for IL1RN and NOS3 to observe repeat numbers. Compared to homozygous wild type
(C/C) variant, heterozygous (C/A) and homozygous mutant (A/A) genotypes have shown
increased risk (OR=3.18; 95% CI–1.02-9.91; p=0.04; OR=3.30; 95% Cl=0.98–11.07;
p=0.05) of IUFD in PEMT analysis. However, the PEMT C/A and A/A genotypes were
found to be associated with IUFD risk, in cases with no previous use of birth control
(OR=0.27; 95% CI=0.08-0.92; p=0.04 and OR=0.02; 95% CI=0.06–0.79; p=0.02
respectively). IL1RN (Allele b/b) exhibited increased risk for IUFD when interacted with
PEMT (A/A) genotype by 2 folds (OR=2; 95% CI=0.15–26.73; p=0.05). The genotypes of
CHKA did not show any significant increase in IUFD risk. Interestingly, MTHFR and NOS3
manifested uniform distribution among cases and controls suggesting no association with
IUFD risk in the Bangladeshi population.