dc.contributor.advisor | Ahmed, Akash | |
dc.contributor.author | Sarah, Humaira Reza Khan | |
dc.contributor.author | Faiza, Refat Rahman | |
dc.date.accessioned | 2024-06-12T05:51:57Z | |
dc.date.available | 2024-06-12T05:51:57Z | |
dc.date.copyright | 2023 | |
dc.date.issued | 2023-08 | |
dc.identifier.other | ID 16204088 | |
dc.identifier.other | ID 19326027 | |
dc.identifier.uri | http://hdl.handle.net/10361/23391 | |
dc.description | This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023. | en_US |
dc.description | Catalogued from the PDF version of thesis. | |
dc.description | Includes bibliographical references (pages 34-40). | |
dc.description.abstract | Introduction: The main purpose of this study is to identify the multidrug-resistant genes from
some specific microorganisms. Among them, our selected organism was Pseudomonas
aeruginosa. The highly known Carbapenem and beta-lactamase-producing Pseudomonas
aeruginosa was given priority in this study to test how much resistance and sensitivity is related
to this organism in the context of Bangladesh, particularly in the highly polluted and unhealthy
conditions of Dhaka city. To conduct this study different water samples from hospital waste and
its surrounding selected community tap has been collected. Hospital wastewater is one of the
major sources of antibiotic-resistant pathogens that is becoming an alarming concern daily.
Method: This study has been from January 2023 to June 2023. A total of 54 confirmed
Pseudomonas aeruginosa isolates were suspected from 78 isolates collected from hospital
wastewater and adjacent community tap water. To identify exact isolates of Pseudomonas
aeruginosa, the phenotypic technique was used. Also, the Antibiotic Susceptibility test helped to
detect which antibiotics from different generations are susceptible or resistant against the specific
isolate. Since biochemical procedures are less accurate than PCR and need additional resources
and time besides these methods, we did further PCR tests to identify the resistant gene of ESBL
(Extended spectrum β-lactamase) and MBLS (Metallo-beta-lactamase) after getting the
multidrug-resistant isolates. ATCC strains are used as positive controls.
Result: There was a total of 78 samples taken from the nearby community water and the three
hospitals, and 54 of those 78 samples contained Pseudomonas aeruginosa isolates that could be
confirmed. There are 69.23% confirmed isolates. These 54 confirmed isolates showed resistance
to different 12 antibiotics. The hospital water showed the most resistance towards amoxyclav
(94%) and then towards cefixime and ceftriaxon (82%). Whereas, the community water isolates
resistance was quite similar. The highest resistance they showed towards amoxiclav (90%) and
the second highest is cefixime (82%). Among all these isolates 7 isolates were chosen that
showed the most resistance and further identification of Multidrug Resistance were done. Among
them, for Bla-CtxM and Bla-SHV, two isolates from the June sample had positive results, or 28.5% of the total and one isolate from the June sample tested positive for Bla-oxa 48, or 14.2%
of the total.
Conclusion: The quality of drinking water, distribution lines, and waste management of hospital
water have now become key concerns for the spread of antibiotic resistance among various
species, particularly for Pseudomonas aeruginosa. So, the goal of this study is to find out if this
multi-drug-resistant bacteria is present in the wastewater of the hospital and its adjacent
community water. | en_US |
dc.description.statementofresponsibility | Humaira Reza Khan Sarah | |
dc.description.statementofresponsibility | Refat Rahman Faiza | |
dc.format.extent | 40 pages | |
dc.language.iso | en | en_US |
dc.publisher | Brac University | en_US |
dc.rights | Brac University dissertations are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. | |
dc.subject | Pseudomona | en_US |
dc.subject | Antimicrobial resistant | en_US |
dc.subject | Wastewater | en_US |
dc.subject.lcsh | Biomolecules--Analysis. | |
dc.title | “Pseudomonas aeruginosa characterization and analysis based on its antimicrobial resistant pattern collected from hospital wastewater and adjacent community water.” | en_US |
dc.type | Dissertation | en_US |
dc.contributor.department | Department of Mathematics and Natural Sciences, Brac University | |
dc.description.degree | B. Microbiology | |