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dc.contributor.advisorNaser, Iftekhar Bin
dc.contributor.authorAfrid, Abir Hassan
dc.date.accessioned2024-06-03T04:07:13Z
dc.date.available2024-06-03T04:07:13Z
dc.date.issued2023-10
dc.identifier.otherID 19136005
dc.identifier.otherID 19136049
dc.identifier.urihttp://hdl.handle.net/10361/23070
dc.descriptionThis thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023.en_US
dc.descriptionCatalogued from PDF version of thesis.
dc.descriptionIncludes bibliographical references (pages 93-106).
dc.description.abstractThis study meticulously probes the impact of both isogenic and heterogenic genomic DNA, in its entirety and fragmented states, on the density of biofilms formed by distinct bacterial strains, spanning both gram-positive and gram-negative classifications. Utilising samples from two distinct strains of Vibrio cholerae (WT346 and 1877), Escherichia coli (0157:H7), and Staphylococcus aureus (ATCC 25923), which were procured from the Life and Natural Sciences Laboratory at BRAC University, the formation and robustness of biofilms were quantitatively analysed via the crystal violet assay technique. Notably, biofilm density exhibited pronounced variations when exposed to isogenic genomic DNA as opposed to its heterogenic counterpart. Moreover, intact genomic DNA consistently elicited enhanced biofilm thickness relative to its fragmented variant, which elicited a more heterogeneous and attenuated response. Among the bacterial exemplars scrutinised, strain 1877 exhibited the most pronounced percentage augmentation in biofilm density in the presence of both fragmented (150%) and whole (125%) ATCC 25923 genomic DNA. This investigation underscores the nuanced interplay between genomic DNA and bacterial biofilm dynamics, offering pivotal insights for microbial ecology.en_US
dc.description.statementofresponsibilityAbir Hassan Afrid
dc.description.statementofresponsibilityMohammad Rafiue Rahman
dc.format.extent118 pages
dc.language.isoenen_US
dc.publisherBrac Universityen_US
dc.rightsBrac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission.
dc.subjectBiofilm formationen_US
dc.subjectGenome DNAen_US
dc.subjectIsogenicen_US
dc.subjectHeterogenicen_US
dc.subject.lcshBiofilms--growth & development
dc.subject.lcshGene mapping
dc.titleUnveiling the role of free DNA in biofilm formation; potential relevance in Cholera epidemiologyen_US
dc.typeThesisen_US
dc.contributor.departmentDepartment of Mathematics and Natural Sciences, Brac University
dc.description.degreeB. Biotechnology


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