A comparative study on the antimicrobial resistance pattern of Klebsiella pneumoniae isolated from hospital wastewater and its surrounding community

Abstract

Klebsiella pneumoniae (Kpn) is a member of the Enterobacteriaceae which is a gram-negative, encapsulated, and non-motile bacterium. K. pneumoniae spreads a range of infections, including pneumonia, urinary tract infections and liver abscesses. K. pneumoniae is an important microorganism because it shows high resistance to carbapenems and beta- lactams. It can produce carbapenemase and extended spectrum beta-lactamase to resist this group of antibiotics. For that reason, is organism has a significant role in highly prevalent infections which are very difficult to treat. Our study was to collect samples from three different hospitals situated in Dhaka north city corporation. Samples were collected in two categories; one sample is collected from the hospital effluents and another sample was collected from the adjacent communities of that hospitals. Then, some isolates were collected from the hospital wastewater and some isolates were collected from the community water. Then, from those isolates, K. pneumoniae were confirmed through PCR. Then AST was done to observe the AMR pattern of the isolates. From 177 isolates, 119 isolates were confirmed as Klebsiella pneumoniae which is 67% of the total isolates. In March, highest number of isolates were detected which is 30.25% of the total confirmed isolates. From Dhaka Shishu Hospital, greatest number of isolates were obtained which is 42%. Antibiotic susceptibility test was done, and the result founded that the isolates showed highest sensitivity to gentamicin (90%) and highest resistance to erythromycin (98%). Both the isolates from hospital wastewater and community water showed resistance to multiple antibiotics. Tests for AMR-genes were run and BlaCTXM, BlaSHV, BlaTEM, BlaNDM gene were found. BlaCTXM was found in most of the isolates which is 40%. BlaSHV, BlaTEM, BlaNDM these three genes were found in almost equal amount which is 20% each.