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dc.contributor.advisorNaser, Iftekhar Bin
dc.contributor.authorRahman, Wasifa Ar
dc.date.accessioned2024-06-02T04:18:00Z
dc.date.available2024-06-02T04:18:00Z
dc.date.copyright©2023
dc.date.issued2023-07
dc.identifier.otherID 21276003
dc.identifier.urihttp://hdl.handle.net/10361/23039
dc.descriptionThis thesis is submitted in partial fulfillment of the requirements for the degree of Masters of Science in Biotechnology, 2023.en_US
dc.descriptionCatalogued from PDF version of thesis.
dc.descriptionIncludes bibliographical references (pages 22-24).
dc.description.abstractBacterial gene transfer is responsible for spread of antibiotic resistance in bacteria and evolution of bacteria which can be a threat of virulence as well play role in maintenance. Horizontal gene transfer (HGT) is the key process for transferring gene. It involves three mechanisms- conjugation, transformation and transduction. To distantly related species of bacteria, via HGT, exogenous DNA can be transferred from one bacterium to another. Apart from HGT, prophage induction and infection can be a way of transferring gene. For this, the prophage is induced by UV radiation and infects with bacterial competent cell to make the new viral particles. On the infection with other bacteria, the prophage can carry the gene through plasmid packaging or miss packaging and may also be integrated to the chromosomal DNA of the newly formed particle. Following that, in preliminary selection it showed growth of resistant colonies of its bacteria insuring of gene transfer. Further for genetic validation, plasmid isolation assures of plasmid packaging, here the result was negative for plasmid packaging thus it indicates of gene transferring and integrating to the chromosomal DNA. To validate the result, more advanced study and farther genetic validation is required like polymerase chain reaction to assure the presence of the antibiotic resistant gene in the infected particles.en_US
dc.description.statementofresponsibilityWasifa Ar Rahman
dc.format.extent39 pages
dc.language.isoenen_US
dc.rightsBrac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission.
dc.subjectBacterial gene transferen_US
dc.subjectPlasmidsen_US
dc.subjectAMR genesen_US
dc.subjectHorizontal gene transfer (HGT)en_US
dc.subject.lcshBacterial genetic engineering
dc.subject.lcshPlasmids
dc.subject.lcshBacterial spores
dc.titleCorrelation between the prevalence of plasmids and AMR genes in the environmental isolates of different bacterial speciesen_US
dc.typeThesisen_US
dc.contributor.departmentDepartment of Mathematics and Natural Sciences, Brac University
dc.description.degreeB. Biotechnology


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