dc.contributor.advisor | Ahmed, Akash | |
dc.contributor.author | Tahmid, Md. Izhar | |
dc.contributor.author | Siddiqua, Iritra | |
dc.date.accessioned | 2024-01-04T08:53:01Z | |
dc.date.available | 2024-01-04T08:53:01Z | |
dc.date.copyright | 2022 | |
dc.date.issued | 2022-08 | |
dc.identifier.other | ID: 18136080 | |
dc.identifier.other | ID: 18136005 | |
dc.identifier.uri | http://hdl.handle.net/10361/22071 | |
dc.description | This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022. | en_US |
dc.description | Catalogued from PDF version of thesis. | |
dc.description | Includes bibliographical references (pages 66-73). | |
dc.description.abstract | Keratin rich waste by-products from animal skin and other parts are being produced every
year in a huge amount. These wastes are required to be degraded. Otherwise, it can become a
hazardous to the environment. This study focuses to find potential bacteria from the soil
sample of leather manufacturing industry located at Hazaribagh of Dhaka City, which can
produce keratinase enzyme to degrade the keratin wastes. 16 individual colonies of bacteria
were selected to observe the keratinolytic activity of those bacteria. Those bacteria were
inoculated in minimal salt media and tested with chicken feathers onto it and results were
observed with the changes in the smoothness and degradation of the feather. Only 8 bacterial
colonies showed changes after keeping the solution in rotatory shaker incubator. Changes in
the feather were assumed to be keratin degradation. To verify the degradation of keratin, the
absorbance of the optical density of keratinolytic assay was observed of those bacteria.
Different biochemical tests were done for the identification of the bacteria and the process
was done with different temperature and pH separately for optimization of the experiment.
Finally, extraction of DNA of those bacteria was done, along with the PCR (Polymerase
Chain Reaction) to get the potential bacteria for further research. | en_US |
dc.description.statementofresponsibility | Md. Izhar Tahmid | |
dc.description.statementofresponsibility | Iritra Siddiqua | |
dc.format.extent | 73 pages | |
dc.language.iso | en | en_US |
dc.publisher | Brac University | en_US |
dc.rights | Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. | |
dc.subject | Keratin | en_US |
dc.subject | Minimal salt media | en_US |
dc.subject | Keratinolytic assay | en_US |
dc.subject | Soil sample | en_US |
dc.subject | Biochemical tests | en_US |
dc.subject | Bacillus licheniformis | en_US |
dc.subject | Bacillus cereus | en_US |
dc.subject | Bacillus carboniphilus | en_US |
dc.subject | Serratia marcescens | en_US |
dc.subject.lcsh | Biotechnology. | |
dc.subject.lcsh | Chemistry. | |
dc.title | Isolation, identification, and characterization of keratinase enzyme producing bacteria, collected from tannery soil | en_US |
dc.type | Thesis | en_US |
dc.contributor.department | Department of Mathematics and Natural Sciences, Brac University | |
dc.description.degree | B. Biotechnology | |