| dc.contributor.advisor | Choudhury, Naiyyum | |
| dc.contributor.advisor | Ahmed, Monzur Morshed | |
| dc.contributor.author | Quayum, Md. Abdul | |
| dc.date.accessioned | 2012-03-06T03:28:45Z | |
| dc.date.available | 2012-03-06T03:28:45Z | |
| dc.date.copyright | 2011 | |
| dc.date.issued | 2011-11 | |
| dc.identifier.other | ID 10376003 | |
| dc.identifier.uri | http://hdl.handle.net/10361/1651 | |
| dc.description | This thesis report is submitted in partial fulfillment of the requirement for the degree of Masters of Science in Biotechnology, 2011 | |
| dc.description | Cataloged from PDF version of thesis report. | |
| dc.description | Includes bibliographical references (page 55-60). | |
| dc.description.abstract | Bioethanol or biofuel as an alternative to fossil fuels has been expanded in the last few decades
in the whole world. Saccharomyces cerevisiae is the choice of organism for ethanol production.
Characterization of an ethanol producing ability and to optimization of ethanol production by a
laboratory strain of yeast was attempted in the present study. Based on morphological and
physiochemical characters, the yeast strain was identified to be Saccharomyces cerevisiae. The
strain was moderately thermotolerant and ethanol tolerant as well as osmotolerant. It was
resistant to cycloheximide at 0.0015g/100ml concentration but growth was inhibited in presence
of Hydrogen peroxide. Ethanol producing capability of the Saccharomyces cerevisiae strain was
studied using sugarcane molasses as substrate. The reducing sugar was estimated by DNS
method and ethanol was estimated by redox titration. Fermentation was optimized with respect to
temperature, reducing sugar concentration and pH. Analysis of fermentation characteristics under
different substrate and environmental conditions, it was observed that reducing sugar
concentration of 5%-6%, temperature of 30°Cand pH 6.0 were optimum for fermentation with
maximum yield of ethanol. Maximum ethanol production was 11 % by free cells using the initial
reducing sugar concentration 5.50% at 48 hrs under shaking condition. Immobilized yeast cells
showed significant improvement of ethanol production. Under shaking condition, 12% ethanol
was produced by immobilized cells using the reducing sugar concentration 6% at 48 hrs. Ethanol
production was higher in shaking condition than that in stationery non shaking condition using
the same environmental condition. Influence of boron, chromium, copper, magnesium chloride
was investigated on ethanol production. Only chromium was found to show stimulatory effect on
ethanol production. This selected strain could be potential strain for ethanol production from
cane molasses. | en_US |
| dc.description.statementofresponsibility | Md. Abdul Quayum | |
| dc.format.extent | 65 pages | |
| dc.publisher | BRAC University | en_US |
| dc.rights | BRAC University thesis are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. | |
| dc.subject | Biotechnology | |
| dc.title | Characterization of an ethanol producing yeast isolate and optimization of ethanol fermentation | en_US |
| dc.type | Thesis | en_US |
| dc.contributor.department | Department of Mathematical and Natural Science, BRAC University | |
| dc.description.degree | M. Biotechnology | |
