An in-vitro study on antioxidant properties of Alcea rosea leaves

Abstract

The aim of the study is to determine free radical scavenging capacity, total phenolic content and total flavonoid content and to investigate the in-vitro antioxidant potential of methanolic leaves extract of Alcea rosea. The free radical scavenging capacity, TPC and TFC were performed using DPPH scavenging assay, Folin-Ciocalteu reagent assay and aluminium chloride colorimetric assay respectively. The result of the study indicates that Alcea rosea posses significant scavenging activity against DPPH and result is very much similar to standard. The IC50 value of the sample was 255.13 ± 8.35 μg/mL and comparable with the standard 167.84 ± 5.11 μg/mL. The study of total phenolic content and total flavonoid content revealed that the leaves of Alcea rosea has significant amount of phenolic content 52.2 ± 1.1 mg GAE/g and flavonoid content 29.4 ± 0.707 mg QE/g. The obtained results suggest that Alcea rosea exhibits antioxidant activity. But further studies are needed to validate its medicinal uses.