| dc.description.abstract | Feathers, horns, cows skin are by product waste of cows skin, poultry and skin processing
plants and produced in huge amount of waste every year. This horns, skin hair, chicken feather
waste can be modified to make animal dietary protein. This conversion can be done by
Keratinolytic bacteria, High protein content of keratin waste can be used as a good source of
protein and amino acids by systemic recycling. Feather recycling can provides a cheap and
alternative protein feed stuff. Further this can be used for many other purposes more likely
animals feed fish feed. Some Bacteria, Fungi and Actinomycetes have been reported to produce
keratinase in the presence of keratin substrate. In the present study Keratinase producing
bacteria was isolated from soil and their Keratinolytic enzyme production was investigated.
Soil sample was collected from Hazaribagh skin processing site Dhaka, Bangladesh, an organic
waste dumping site. Soil samples were inoculated in eight nutrient broth media enriched with
hair horns, feather powder and inoculated bacteria was isolated from soil sample. Only two
organisms were able to degrade hair horns and feather powder indicating that these isolates
produced Keratinase enzyme. In this study it was observed that keratinase and protease activity
were detected in the culture supernatant and optimal medium for extracellular production of
keratinase and protease is Keratin powder milk media at pH (7.5) and temperature (37oC).
There was complete degradation of keratin after 168 hour of incubation in rotary shaking
incubator 150 RPM at 37oC. Finally, biochemical test was done to identify the organism and it
was found to be Bacillus licheniformis. | en_US |
