Isolation of bacteria from dairy-based popular sweetmeat named swandesh following the molecular identification of Bacillus species

Abstract

Among all the sweetmeats found in Bangladesh, Swandesh achieves an intense popularity due to its delicate texture and sublime taste. Like other dairy products, it is high in carbohydrates and proteins, along with probiotic contents. However, the presence of pathogenic bacteria in Swandesh put them at a grave risk for public health. Therefore, it is highly crucial to evaluate the microbial contamination in it. Current study was done to check the status of several microorganisms in Swandesh. After isolation and identification, the most prevalent microorganism in Swandesh was found out as Staphylococcus species followed by Bacillus species. The samples were then demographically represented which showed Mohakhali and Dhanmondi having the highest number of microbial isolates. Later, the antibiotic sensitivity testing was done to check the effectivity of antibiotics against the isolates. Where ciprofloxacin was effective against 100% of the isolates. On the other hand, all the isolates were resistant of cefepime. The isolates of Bacillus which showed the highest amount of resistance, were taken for PCR and both samples gave bands at about 1500 bp, which corresponds to their band size for 16s rRNA amplification. This shows the effectivity of the PCR method for rapidly detecting Bacillus from food samples.