Article
http://hdl.handle.net/10361/6891
2024-03-29T02:36:35Z
2024-03-29T02:36:35Z
In vitro free radical scavenging and thrombolytic activities of Bangladeshi aquatic plant Aponogeton undulatus Roxb
Chowdhury, Nargis S
Alam, Md. Badrul
Haque, A S M Tanbirul
Zahan, Ronok
Ehsanul, M
Mazumder, Haque
Haque, M Ekramul
http://hdl.handle.net/10361/7133
2016-12-06T10:06:21Z
2011-01-01T00:00:00Z
In vitro free radical scavenging and thrombolytic activities of Bangladeshi aquatic plant Aponogeton undulatus Roxb
Chowdhury, Nargis S; Alam, Md. Badrul; Haque, A S M Tanbirul; Zahan, Ronok; Ehsanul, M; Mazumder, Haque; Haque, M Ekramul
Investigation with the crude methanolic extract of Aponogeton undulatus was carried out to evaluate its possible antioxidant and thrombolysis activity. In DPPH free radical scavenging assay, the extract exhibited potent antioxidant activity with a IC50 values of 2.43±1.06 μg/ml while in ascorbic acid, the value become 50 2.14±0.11 μg/ml. In thrombolytic activity using in vitro clot lysis assay method, the crude methanolic extract was found to have significant (p<0.001) thrombolytic activity at a dose of 10 mg/ml with a miximum effect of 20.23±1.56% while the standard streptokinase showed 46.13±3.87%. The extract was also investigated for its antibacterial and toxic potentiality using agar diffusion and Brine Shrimp lethality bioassay, respectively. The highest antibacterial effect was shown against Bacillus cereus (zone of inhibition 12±0.65 mm) followed by Escherichia coli (zone of inhibition 10±0.71 mm). In this bioassay the extract showed significant toxicity to Brine Shrimp nauplii with the LC50 value of 2.24±0.98 μg/ml. The study clearly indicated that the extract 50 possesses good antioxidant and thromolytic activity along with broad spectrum antibacterial and toxic potentiality.
This article was published in Global Journal of Pharmacology [© IDOSI Publications, 2011] and the definite version is available at: https://idosi.org/gjp/5(1)11/6.pdf
2011-01-01T00:00:00Z
Thank you, a good research antioxidant, antimicrobial and toxicity studies of the different fractions of fruits of Terminalia belerica Roxb
Alam, Md. Badrul
Zahan, Ronok
Hasan, Mahedi
Maruf, M
Khan, Md. Maruf
S Rahman, Nargis
Chowdhury, M
Haque, Ekramul
http://hdl.handle.net/10361/7131
2016-12-06T09:29:54Z
2011-01-01T00:00:00Z
Thank you, a good research antioxidant, antimicrobial and toxicity studies of the different fractions of fruits of Terminalia belerica Roxb
Alam, Md. Badrul; Zahan, Ronok; Hasan, Mahedi; Maruf, M; Khan, Md. Maruf; S Rahman, Nargis; Chowdhury, M; Haque, Ekramul
The crude methanolic extract of the fruits of Terminalia belerica Roxb. along with its various organic fractions were tested to evaluate the toxicity, antioxidant (in vitro and in vivo) and antibacterial activity. The different assay methods, including total antioxidant activity, scavenging free radical, authentic peroxynitrite and reducing power assessment were used to evaluate the antioxidant potential of the crude extract and its organic fractions. While antimicrobial activities were evaluated using agar disc diffusion method and measuring their MIC value. The EtOAc fraction, showed strong activity in all the model systems tested and in peroxynitrite model this fraction (IC50= 1.29±0.18 μg/ml) exerted four-fold stronger activity than standard penicillamine (IC50 value of 5.20±0.32 μg/ml). The reducing power of the extract was found to be concentration dependent. 50 The administration of the extract/fractions at a dose of 200 and 400 mg/kg body weight to the male Wistar rats increased the percentage inhibition of reduced glutathione, superoxide dismutase and catalase significantly. Whereas, lipid peroxidation level in hepatotoxic rats markedly decreased at a dose of 400 mg/kg body weight after 7 days. EtOAc fractions showed also the highest antibacterial effect against Staphylococcus aureus with zone of inhibition 30.00±0.25 mm. The Brine shrimp lethality bioassay was used to determine the toxicity of the extracts where dichloromethane (CH2 Cl2) fraction showed highest activity (LC50= 39.02±1.16 μg/ml). In conclusion, the study clearly indicated that the extract/fractions of Terminalia belerica Roxb possesses good antioxidant and antimicrobial activity along with moderate toxicity.
This article was published in Global Journal of Pharmacology [© IDOSI Publications, 2011] and the definite version is available at: http://idosi.org/gjp/5(1)11/3.pdf
2011-01-01T00:00:00Z
Antioxidant, analgesic and anti-inflammatory activities of the herb Eclipta Prostrate
Hossain, M.S.
Alam, M.B.
Chowdhury, N.S.
Asadujjaman, M.
Zahan, R.
Islam, M.M.
Mazumder, M.E.H.
Haque, M.E.
Islam, A.
http://hdl.handle.net/10361/7126
2016-12-06T07:37:22Z
2011-01-01T00:00:00Z
Antioxidant, analgesic and anti-inflammatory activities of the herb Eclipta Prostrate
Hossain, M.S.; Alam, M.B.; Chowdhury, N.S.; Asadujjaman, M.; Zahan, R.; Islam, M.M.; Mazumder, M.E.H.; Haque, M.E.; Islam, A.
The present study was designed to investigate the antioxidant, analgesic and anti-inflammatory potential of the methanolic extract along with its organic soluble fractions of the herb Eclipta prostrata. In addition, total phenolic and flavonoid content and total antioxidant capacity were also determined. Antioxidant potential of the extract/fractions was evaluated by DPPH (1,1diphenyl-2-picrylhydrazyl), NO (nitric oxide) and ONOO- (peroxynitrite) scavenging assay method. Ethyl acetate fractions (EtOAc) showed highest scavenging activity in all the methods with IC50 value of 12.98±0.08, 45.98±0.07 and 14.45±0.18 μg mL-1 for DPPH, NO and ONOO- assay method, respectively. In reducing power assay, EtOAc fraction also showed significant (p<0.001) activity. Further, the extract/fractions were studied for their analgesic (hot plate, tail immersion and acetic acid induced writhing test) and anti-inflammatory (carrageenan induced paw edema in rats) activities at a dose level of 200 and 400 mg kg-1 body weight. Among all the extract/fractions, EtOAc fraction showed a dose dependent and significant (p<0.005, p<0.05) analgesic activity in all the tested method. EtOAc fractions also reduced the paw edema considerably (86.80% inhibition after 3 h, p<0.005, p<0.05) in dose dependent manner compared to carrageenan induced rat. Altogether, these results suggest that the MeOH extract and its organic soluble fractions could be used as a potential antioxidant, analgesic and anti-inflammatory agent
This article was published in Journal of Pharmacology and Toxicology [© 2011 Academic Journals Inc.] and the definite version is available at: http://docsdrive.com/pdfs/academicjournals/jpt/0000/28817-28817.pdf
2011-01-01T00:00:00Z
Antioxidant, anti-inflannnatory and anti-pyretic activities of trichosanthes dioica roxb. fruits
Alam, M. Badrul
Hossain, M. Sarowar
Chowdhury, N. Sultana
Asadujjaman, M.
Zahan, Ronok
Islam, M. Monirul
Mazumder, M. Ehsanul Haque
Haque, M. Ekramul
Islam, Anwarul
http://hdl.handle.net/10361/7123
2018-04-23T10:57:50Z
2011-01-01T00:00:00Z
Antioxidant, anti-inflannnatory and anti-pyretic activities of trichosanthes dioica roxb. fruits
Alam, M. Badrul; Hossain, M. Sarowar; Chowdhury, N. Sultana; Asadujjaman, M.; Zahan, Ronok; Islam, M. Monirul; Mazumder, M. Ehsanul Haque; Haque, M. Ekramul; Islam, Anwarul
The present study was designed to investigate the antioxidant, anti-inflammatory and antipyretic potential of the methanolic extract along with its organic soluble fractions of the fruits of Trichosanthes dioica Roxb. Antioxidant potential of the extract/fractions was evaluated by DPPH (1,ldiphenyl-2-picrylhydrazyl) and NO (nitric oxide) scavenging assay method. Ethyl acetate fractions (EtOAc) showed highest scavenging activity in all the methods with IC50 value of 12.32±0.16 and 5.38±0.07 pg mL -1 for DPPH and NO assay method, respectively. In reducing power assay, EtOAc fraction also showed significant (p<O.OOl) activity. Further, the extract/fractions were studied for their anti-inflammatory (carrageenan induced paw edema in rats) and antipyretic (Brewer's yeast induced pyrexia) activities at a dose level of 100, 200 and 400 mg kg-1 body weight for MeOH extract. Methanolic extract showed a dose dependent and significant (p<0.005, p<0.05) anti -inflammatory and antipyretic effect. Dichloromethane fraction (CH2CI2) and Ethyl acetate fractions exhibited similar activity using a dose of 200 mg kg-1 b.wt. in these models. The pharmacological activities of the (CH2CI2) fraction were lesser than the MeOH extract and other fractions. In addition, total phenolic and flavonoid content and total antioxidant capacity were also determined. Altogether, these results suggest that the MeOH extract and its organic soluble fractions EtOAc could be used as a potential antioxidant, anti -inflammatory and antipyretic agent.
This article was published in Journal of Pharmacology and Toxicology [© 2011 Academic Journals Inc.] and the definite version is available at: https://goo.gl/yQ4Sfd
2011-01-01T00:00:00Z