Laizuman Nahar
http://hdl.handle.net/10361/6765
2024-03-28T19:35:11Z
2024-03-28T19:35:11Z
Antioxidant, analgesic and CNS depressant effects of Synedrella nodiflora
Nahar, Laizuman,
Zahan, Ronok
Ibn Morshed, Mahmud Tareq
Haque, Anamul
Alam, Zahangir
Mosaddik, Ashik
http://hdl.handle.net/10361/7011
2016-12-04T08:23:40Z
2016-10-01T00:00:00Z
Antioxidant, analgesic and CNS depressant effects of Synedrella nodiflora
Nahar, Laizuman,; Zahan, Ronok; Ibn Morshed, Mahmud Tareq; Haque, Anamul; Alam, Zahangir; Mosaddik, Ashik
Synedrella nodiflora (L) Gaertn. belonging to the family Asteraceae contains steroids, reducing sugars, phenolic, flavonoids, saponins, tannins and triterpenoids. Therefore, this study is designed to investigate its antioxidant, analgesic and CNS depressant effects. Materials and Methods: The antioxidant activity of methanolic extract of S. nodiflora (SN) was determined using Folin Ciocalteu reagent, phosphomolybdenum method, free radical scavenging activity by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and the reducing power activity. Analgesic activity was done by acetic acid and formalin model and CNS depressant activity was measured by the hole cross and open field method. Results: The total phenols and total antioxidant capacity of SN was found to be 37.38 < 2.01 mg/g equivalent of gallic acid and 433.37 < 4.22 mg/gm equivalent of ascorbic acid. The percentage (%) scavenging of DPPH free radical was found to be concentration dependent and IC50 value was 125.89 < 2.23 µg/ml while the IC50 value of standard ascorbic acid was found to be 16.76 < 0.11 µg/ml. The reducing power of SN was found to be concentration dependent. Acetic acid and formalin models are peripherally acting analgesic methods. The oral administration of both doses (100 and 200 mg/kg b.wt.) of SN significantly (p<0.001) inhibited 40.06% and 61.83% writhing response induced by acetic acid whereas oral administration of the same doses of SN significantly (p<0.001) inhibited 56.96% and 62.60% itching response induced by formalin. The methanolic extract of SN, at the dose of 250 mg/kg and 500 mg/kg b.wt. produced significant (P<0.001) decrease of locomotion. This is the first report of CNS depressant activity of the plant.
This article was published in Pharmacognosy Journal [© 2012 Elsevier Ltd.] and the definite version is available at http://www.sciencedirect.com/science/article/pii/S0975357512800824 The article website is at: http://www.sciencedirect.com/science/journal/09753575/4
2016-10-01T00:00:00Z
Comparative study of antidiabetic activity of Cajanus cajan and Tamarindus indica in alloxan-induced diabetic mice with a reference to in vitro antioxidant activity
Nahar, Laizuman
Haque, Ekramul
Nasrin, Fatema
Mosaddik, Ashik
Zahan, Ronok
Haque, Anamul
http://hdl.handle.net/10361/6825
2016-12-04T08:25:40Z
Comparative study of antidiabetic activity of Cajanus cajan and Tamarindus indica in alloxan-induced diabetic mice with a reference to in vitro antioxidant activity
Nahar, Laizuman; Haque, Ekramul; Nasrin, Fatema; Mosaddik, Ashik; Zahan, Ronok; Haque, Anamul
Oxidative stress not only develops complications in diabetic (type 1 and type 2) but also contributes to beta cell destruction in type 2 diabetes in insulin resistance hyperglycemia. Glucose control plays an important role in the pro-oxidant/antioxidant balance. Some antidiabetic agents may by themselves have antioxidant properties independently of their role on glucose control. Objective: The present investigation draws a comparison of the protective antioxidant activity, total phenol content and the antihyperglycemic activity of the methanolic extract of Cajanus cajan root (MCC) and Tamarindus indica seeds (MTI). Materials and Methods: Antidiabetic potentials of the plant extracts were evaluated in alloxan-induced diabetic Swiss albino mice. The plant extracts at the doses of 200 and 400 mg/kg body weight was orally administered for glucose tolerance test during 1-hour study and hypoglycemic effect during 5-day study period in comparison with reference drug Metformin HCl (50 mg/kg). In vitro antioxidant potential of MCC and MTI was investigated by using 1, 1- diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity at 517 nm. Total phenolic content, total antioxidant capacity and reducing power activity was also assayed. Results: There was a significant decrease in fasting serum glucose level (P < 0.001), reduction in blood glucose level (P < 0.001) in 5-days study, observed in the alloxan-induced diabetic mice. The reduction efficacy of blood glucose level of both the extracts is proportional to their dose but MCC is more potent than MTI. Antioxidant study and quantification of phenolic compound of both the extracts revealed that they have high antioxidant capacity. Conclusion: These studies showed that MCC and MTI have both hypoglycemic and antioxidant potential but MCC is more potent than MTI. The present study suggests that both MCC and MTI could be used in managing oxidative stress.
This article was published in Pharmacognosy Research [© 2014 Pharmacognosy Network Worldwide] and the definite version is available at: http: http://www.phcogres.com/article.asp?issn=0974-8490;year=2014;volume=6;issue=2;spage=180;epage=187;aulast=Nahar The article website is at: http://www.phcogres.com