Department of Mathematics and Natural Sciences (MNS)http://hdl.handle.net/10361/1062024-03-29T15:03:39Z2024-03-29T15:03:39ZIsolation and Characterization of E. coli Bacteriophage from Raw Meat and Environment WaterIslam, MahbubaSiddik, Md Abu BakarMondol, SudorshonHossain, Md Shahriarhttp://hdl.handle.net/10361/224822024-03-13T03:03:13Z2023-12-01T00:00:00ZIsolation and Characterization of E. coli Bacteriophage from Raw Meat and Environment Water
Islam, Mahbuba; Siddik, Md Abu Bakar; Mondol, Sudorshon; Hossain, Md Shahriar
Several diseases such as stomach cramps, urinary tract infections, and diarrhea are caused by the bacterium Escherichia coli. One of the five subtypes of E. coli which are ETEC, EPEC, EAEC, EHEC/STEC, and EIEC are responsible for intestinal infections. E. coli outbreak is a major public health concern. One of the key elements that can put an end to outbreaks could be bacteriophages. Since their discovery in 1915, they have been beneficial to humans in a variety of ways, including phage therapy, genetic screening tools, diagnostic weaponry, pathogenic bacteria detectors, therapeutic agents, and more. Phage research has gained attention because of the recent rise in antibiotic resistance because of their capacity to infect and destroy bacteria without endangering humans. This experiment is designed to isolate and characterize bacteriophage specific to E. coli. It includes procedures that have been used in the lab to check for the presence of phages and the results of the experiments. In this research, E. coli bacteria and bacteriophage was isolated from the Raw meat. Unfortunately, only one bacteriophage was possible to isolate from Raw meat and which is why water samples are collected from various regions of Mohakhali, Gulshan and Hatirjheel and from the samples, four more bacteriophages were successfully isolated. Phage ECPW4.4, ECPW5.1 and ECPW12.3 were chosen for further analysis and characterization. In characterization, three tests were done, which were; Temperature test, salinity test and pH test. On these three tests, the three bacteriophages showed better results overall. Furthermore, more tests and analysis are needed to be done but because of the closure of BRAC University Lab, further research was not possible.
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology and Bachelor of Science in Microbiology, 2023.; Catalogued from PDF version of thesis.; Includes bibliographical references (pages 62-65).
2023-12-01T00:00:00ZAssociation between the severity of helicobacter pylori infection and different hematological parametersAbreshmi, Afrahttp://hdl.handle.net/10361/221802024-01-17T21:02:42Z2023-01-01T00:00:00ZAssociation between the severity of helicobacter pylori infection and different hematological parameters
Abreshmi, Afra
Helicobacter pylori infection is a global public health problem that is affecting both developed
and developing countries. Approximately 50% (over 3 billion) of the world's population is infected
with Helicobacter pylori, mainly in developing countries. H. pylori is a gram-negative bacterium
that is commonly found in the stomach. The vast majority of H.pylori-infected people show no
symptoms and never develop any problems. However, this bacterium is capable of causing ulcers
and less commonly stomach cancer. Recently, its association with some hematological
abnormalities has been found. Compared to other developing countries, in Bangladesh, the rate of
H. pylori infection is particularly high. However, limited studies have been conducted regarding
the relationship between hematological parameters with H. pylori infection in Bangladesh. Thus,
the objective of our study was to determine the correlation between different selected
hematological parameters and H. pylori infections of different intensities. A total of 697 patients,
290 males, and 407 females, who were suspected H. pylori patients, were included in the study.
The suspected patients infected with H. pylori titer less than 30 U/ml were considered H. pylori negative and more than 30 U/ml were under H. pylori-positive group. Patients in H. pylori-positive
group were divided into a subgroup of severe intensity (titer more than 50 U/ml). Our study found
a weak positive correlation between Lymphocyte and H.pylori (p= 0.0253*) and between
Neutrophil to Platelet ratio (NPR) and H.pylori (p=0.008*) in the H.pylori-positive group. In the
sub-group of severe intensity, a weak positive correlation between Hemoglobin and H.pylori (p=
0.01126*) and between NPR and H.pylori (p= 0.0362*) was observed. There was a very weak
negative correlation found between Platelet and H.pylori (p= 0.0005*) and between Platelet to
Lymphocyte ratio (PLR) and H.pylori (p= 0.0001*) in the H.pylori-positive group. In the severe
intensity group, a weak negative correlation between Platelet and H.pylori (p= 0.01074*) and
between PLR and H.pylori (p= 0.0034*) was observed.
This thesis report is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2023.; Cataloged from PDF version of thesis.; Includes bibliographical references (pages 25-28).
2023-01-01T00:00:00ZStudy on the numerical analysis of storm surges around the coastal zone of BangladeshJoty, Musarrat Rashidihttp://hdl.handle.net/10361/221552024-01-16T21:02:48Z2023-08-01T00:00:00ZStudy on the numerical analysis of storm surges around the coastal zone of Bangladesh
Joty, Musarrat Rashidi
Storm surges are a natural phenomenon preceding tropical cyclones, taking place in
the coastal zones. Often times they are the most dangerous part of the cyclones,
causing the most amount of damage to the population and property of the affected
area. While it is quite difficult to predict the heights of surges caused by tropical
cyclones accurately in real time, having access to more information regarding surge
levels can help save lives and resources. This study is a review paper focused on
the Bay of Bengal region, specifically the coastal zone of Bangladesh. It contains
derivations of equations and methods as well as definitions of some important aspects
of storm surge prediction models. There is a discussion on numerical modeling
methods of predicting cyclone track, intensity and the associated storm surges along
with the analysis of the data obtained and the results of some relevant papers. The
findings here will hopefully summarize and help interested researchers to gain an idea
on this field and what to focus on for future work. A deeper understanding of such
models can assist governments and relevant authorities in helping communities by
improving their disaster-management strategies in high-risk areas to protect coastal
populations by having accurate, real-time information on upcoming storm surge
forecasts.
This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Mathematics 2023.; Catalogued from PDF version of thesis.; Includes bibliographical references (pages 81-85).
2023-08-01T00:00:00ZFactors associated with antifungal resistance and the recent development in detection & treatment: a reviewMadhuri, Srijilahttp://hdl.handle.net/10361/221512024-01-15T21:02:55Z2023-06-01T00:00:00ZFactors associated with antifungal resistance and the recent development in detection & treatment: a review
Madhuri, Srijila
Antifungal resistance has become a growing concern in recent years, as it has become increasingly difficult to treat fungal infections due to the emergence of drug-resistant organisms. The prevalence of antifungal resistance can be analyzed through various factors, including environmental factors, evolutionary factors, and drug misuse. Environmental factors such as climate change and pollution can affect fungi's growth and survival, leading to resistant strains' development. Evolutionary factors such as genetic mutations and natural selection can also contribute to the emergence of drug-resistant organisms. Additionally, drug misuse, such as overuse or improper use of antifungal medications, can promote the development of resistance. Early detection of antifungal resistance is crucial for effective treatment, and various methods such as susceptibility testing and molecular diagnostics are available for this purpose. In conclusion, the prevalence of antifungal resistance is a complex issue that requires a multifaceted approach to address the various factors contributing to its emergence.
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023.; Catalogued from PDF version of thesis.; Includes bibliographical references (pages 25-28).
2023-06-01T00:00:00Z