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    •   BracU IR
    • School of Data and Sciences (SDS)
    • Department of Mathematics and Natural Sciences (MNS)
    • Bachelor of Science in Microbiology
    • Thesis (Bachelor of Science in Microbiology)
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    Determination of the prevalence and antibiotic susceptibility pattern of the members of Enterobacteriaceae family from the urine samples of UTI suspected patients from a diagnostic center in Dhaka city

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    13126018_MNS.pdf (1.988Mb)
    Date
    2018-03
    Publisher
    BRAC Univeristy
    Author
    Haque, Syeda Samiha
    Metadata
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    URI
    http://hdl.handle.net/10361/9812
    Abstract
    Urinary Tract Infection (UTI) defines a condition in which the urinary tract is infected with a pathogen causing inflammation which is a common, distressing and occasionally life threatening condition. UTI affects people of all ages and both gender. In developing countries, UTI is a common experience in clinical practice. UTI can also lead to bladder infection (cystitis) and kidney. infection (pyelonephritis). Symptoms from a lower urinary tract include pain with urination, frequent urination, and feeling the need to urinate despite having an empty bladder. Symptoms of a kidney infection include fever and flank pain usually in addition to the symptoms of a lower UTI. Rarely the urine may appear bloody. In the very old and the very young, symptoms may be vague or non-specific. Every year millions of people suffer from UTI worldwide, women are mostly affected from this infection. Urinary tract infection is increasing day by day and becoming a life-threatening infection due to its resistance against different antibiotics. Antimicrobial resistance is not only increasing the healthcare costs, but also the severity and death rates from certain infections. In most cases the infectious agents are the members of Enterobacteriaceae family members such as E. coli, Klebsiella spp., Enterobacter spp. and Proteus spp. The aim of this study was to determine the prevalence of the members of Enterobacteriaceae family which are involved in urinary tract infections and the susceptibility of these microorganisms against different antibiotics. 25 urine samples of some UTI suspected male and female patients were collected from a diagnostic center. The samples were plated onto Nutrient agar for total count. Then the isolates were sub-cultured and the selected isolates were grown in MacConkey agar, EMB agar, Hi- Crome agar and Blood agar and Biochemical tests were done for identification of the isolates. About 44 isolates were the members of Enterobacteriaceae family. Klebsiella spp. was predominant pathogen while Enterobacter spp., E. coli and Proteus spp. were also found. Most of the isolates were resistant to Penicillin (90.09%) followed by Erythromycin (84.09%), Ampicillin (75%), Rifampicin (75%) and Cefepime (70.45%), Azithromycin (59.09%), Ciprofloxacin (47.72%) and Tetracycline (31.82%). On the other hand, Streptomycin (2.27%) and Chloramphenicol (9.09%) were found to be effective antibiotics. Considering the above, it is a great matter of concern that the emergence of resistant strains is increasing day by day. However, proper and rational use of antibiotics should be practiced to delay the emergence of the resistant strains.
    Keywords
    Antibiotic
    Description
    This thesis report is submitted in partial fulfilment of the requirement for the degree of B.Sc in Microbiology, 2018.
     
    Catalogued from PDF version of thesis report.
     
    Includes bibliographical references (pages 57-59).
    Department
    Department of Mathematics and Natural Sciences, BRAC University
    Collections
    • Thesis (Bachelor of Science in Microbiology)

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