Abstract:
Urinary Tract Infection (UTI) defines a condition in which the urinary tract is infected with a
pathogen causing inflammation which is a common, distressing and occasionally life threatening
condition. UTI affects people of all ages and both gender. In developing countries, UTI is a
common experience in clinical practice. UTI can also lead to bladder infection (cystitis) and kidney.
infection (pyelonephritis). Symptoms from a lower urinary tract include pain with urination,
frequent urination, and feeling the need to urinate despite having an empty bladder. Symptoms of
a kidney infection include fever and flank pain usually in addition to the symptoms of a lower UTI.
Rarely the urine may appear bloody. In the very old and the very young, symptoms may be vague
or non-specific. Every year millions of people suffer from UTI worldwide, women are mostly
affected from this infection.
Urinary tract infection is increasing day by day and becoming a life-threatening infection due to
its resistance against different antibiotics. Antimicrobial resistance is not only increasing the
healthcare costs, but also the severity and death rates from certain infections. In most cases the
infectious agents are the members of Enterobacteriaceae family members such as E. coli,
Klebsiella spp., Enterobacter spp. and Proteus spp. The aim of this study was to determine the
prevalence of the members of Enterobacteriaceae family which are involved in urinary tract
infections and the susceptibility of these microorganisms against different antibiotics. 25 urine
samples of some UTI suspected male and female patients were collected from a diagnostic center.
The samples were plated onto Nutrient agar for total count. Then the isolates were sub-cultured
and the selected isolates were grown in MacConkey agar, EMB agar, Hi- Crome agar and Blood
agar and Biochemical tests were done for identification of the isolates. About 44 isolates were the
members of Enterobacteriaceae family. Klebsiella spp. was predominant pathogen while
Enterobacter spp., E. coli and Proteus spp. were also found. Most of the isolates were resistant to
Penicillin (90.09%) followed by Erythromycin (84.09%), Ampicillin (75%), Rifampicin (75%)
and Cefepime (70.45%), Azithromycin (59.09%), Ciprofloxacin (47.72%) and Tetracycline
(31.82%). On the other hand, Streptomycin (2.27%) and Chloramphenicol (9.09%) were found to
be effective antibiotics. Considering the above, it is a great matter of concern that the emergence
of resistant strains is increasing day by day. However, proper and rational use of antibiotics should
be practiced to delay the emergence of the resistant strains.
