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Isolation and characterization of bacteriophage from environmental water samples specific for Klebsiella pneumoniae

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dc.contributor.advisor Hossain, Dr. Mahboob
dc.contributor.author Roy, Arka
dc.date.accessioned 2018-01-30T05:29:50Z
dc.date.available 2018-01-30T05:29:50Z
dc.date.copyright 2018
dc.date.issued 2018-01
dc.identifier.other ID 13336006
dc.identifier.uri http://hdl.handle.net/10361/9285
dc.description This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Biotechnology, 2018. en_US
dc.description Cataloged from PDF version of thesis report.
dc.description Includes bibliographical references (pages 72-91).
dc.description.abstract Klebsiella pneumoniae is a clinically important pathogen that is frequently associated with multidrug resistance. Recently, some of its strains have acquired superbug status. Over time it is getting difficult to treat Klebsiella pneumoniae induced infection using antibiotics and it is necessary to identify new approach. Bacteriophages are bacteria-infecting viruses that were previously used to treat various bacterial infection in human. Hence, in this study, Klebsiella pneumoniae specific bacteriophage designated as Klpp1 was isolated and characterized for the first time in Bangladesh in order to develop a potential therapeutic agent. Upon host range examination, Klpp1 phage indicated to have a broad host range within genus Klebsiella since it was able to lyse 8 out of 13 different Klebsiella culture but could not infect bacteria from other genera. The isolated phage was able to survive at a temperature up to 60-degree Celsius and was infective in pH range of pH 4.0 to pH 11. The phage was perfectly stable in chloroform but totally lost its infectivity in ethanol. Klpp1 was found to have a latent period of 20 minutes with a burst size of approximately 140 plaque forming unit per infected cell. Therefore, this study provides an addition to the growing number of bacteriophages isolated, particularly Klebsiella pneumoniae specific bacteriophage. The studies on its biological characteristics may provide useful information and knowledge in establishing potential therapeutic agent against Klebsiella pneumoniae infection. en_US
dc.description.statementofresponsibility Arka Roy
dc.format.extent 94 pages
dc.language.iso en en_US
dc.publisher BRAC Univeristy en_US
dc.rights BRAC University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission.
dc.subject Klebsiella pneumoniae en_US
dc.subject Water en_US
dc.subject Plaque en_US
dc.subject Bacteriophage
dc.subject Bangladesh
dc.subject Klebsiella pneumoniae
dc.subject Host range
dc.subject Heat stability
dc.subject PH stability
dc.subject Chloroform
dc.subject Ethanol
dc.subject Organic solvent
dc.subject Latent period
dc.subject Burst size
dc.subject One step growth
dc.title Isolation and characterization of bacteriophage from environmental water samples specific for Klebsiella pneumoniae en_US
dc.type Thesis en_US
dc.contributor.department Department of Mathematics and Natural Sciences, BRAC University
dc.description.degree B. Biotechnology 


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