• Login
    • Library Home
    View Item 
    •   BracU IR
    • School of Data and Sciences (SDS)
    • Department of Mathematics and Natural Sciences (MNS)
    • Bachelor of Science in Microbiology
    • Thesis (Bachelor of Science in Microbiology)
    • View Item
    •   BracU IR
    • School of Data and Sciences (SDS)
    • Department of Mathematics and Natural Sciences (MNS)
    • Bachelor of Science in Microbiology
    • Thesis (Bachelor of Science in Microbiology)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Rapid identification of Streptococcus pneumoniae strains using 16S rRNA gene based PCR method.

    Thumbnail
    View/Open
    13326004_Bachelor.pdf (2.333Mb)
    Date
    2017-10-26
    Publisher
    BRAC University
    Author
    Tasnim, Sadia
    Metadata
    Show full item record
    URI
    http://hdl.handle.net/10361/9148
    Abstract
    Streptococcus pneumoniae strains are responsible for severe diseases, including pneumonia, bronchitis, otitis media, septicemia, meningitis. Early identification of etiologic agent of pneumococcal diseases is essential and usually performed by “Gold Standard” culture based method which is time-consuming and has poor sensitivity. The aim of this study was to develop a PCR based method for rapid diagnosis of pneumococcal diseases by targeting 16S rRNA region of Streptococcus pneumoniae. In this study, 16S rRNA based primers were designed using CLUSTALW and Primer-BLAST. Appropriate laboratory tests such as optochin disk test, bile solubility test, gram-staining and catalase test as well as PCR, gel run electrophoresis and nucleotide sequencing was performed to examine the specificity and utility of the primers in Streptococcus pneumoniae detection using eighteen stored Streptococcus pneumoniae strains. For further validation, the same primers were used against the DNA extracted from six throat swab samples pre-incubated in enrichment broth and DNA extracted directly from same samples without using enrichment broth. It took total 8 hours and 6 hours respectively to achieve the results. After nucleotide sequencing and subsequent BLASTn analysis, it was substantiated; this approach is specific and faster than present diagnostic approach for Streptococcus pneumoniae detection.
    Keywords
    Streptococcus pneumoniae; Habitat
     
    Description
    This thesis report is submitted in partial fulfillment of the requirement for the degree of B.Sc in Microbiology, 2017.
     
    Cataloged from PDF version of thesis.
     
    Includes bibliographical references (pages xii-xix).
    Department
    Department of Mathematics and Natural Sciences, BRAC University
    Collections
    • Thesis (Bachelor of Science in Microbiology)

    Copyright © 2008-2019 Ayesha Abed Library, Brac University 
    Contact Us | Send Feedback
     

     

    Policy Guidelines

    • BracU Policy
    • Publisher Policy

    Browse

    All of BracU Institutional RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Copyright © 2008-2019 Ayesha Abed Library, Brac University 
    Contact Us | Send Feedback