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dc.contributor.advisorHossain, Dr. Mahboob
dc.contributor.advisorKhan, Trosporsha Tasnim
dc.contributor.authorMahjabeen, Faria
dc.date.accessioned2017-05-07T08:49:00Z
dc.date.available2017-05-07T08:49:00Z
dc.date.copyright2016
dc.date.issued2016-06
dc.identifier.otherID 12126004
dc.identifier.urihttp://hdl.handle.net/10361/8098
dc.descriptionThis thesis report is submitted in partial fulfillment of the requirement for the degree of B.Sc in Microbiology, 2016.en_US
dc.descriptionCataloged from PDF version of thesis report.
dc.descriptionIncludes bibliographical references (page 61-68).
dc.description.abstractCellulases have an escalating demand in many industries as they hold extreme potency for converting the most abundant lignocellulosic material into renewable and sustainable energy, chemicals, fuels and materials. Hence, studies pursue to unfold a novel cellulase that can overcome existing challenges in biorefinery, reduce biofuel production cost, as well as have tremendous applications in industrial processes. Therefore, soil from a dairy farm was screened for potent cellulase producers on CMC agar and the best isolate so far had an extracellular crude enzyme activity of 0.167U/ml and specific activity of 0.333U/mg. The cell morphology, cultural characteristics and biochemical tests revealed the isolate to be facultative anaerobe, gram positive, non motile spore forming rods and presumptively identified it to belong to the genus Bacillus. Later on, molecular analysis was carried out by initially amplifying the 16S rRNA gene of the isolate using universal primers 27F and 1492R vie polymerase chain reaction method. The 16S rRNA gene was further sequenced using Sanger sequencing method which revealed the length of the nucleotide sequence to be 1381bp. The sequence was compared to the NCBI nucleotide database. Afterwards a phylogenetic tree was constructed by maximum likelihood method. The organism was found to be Bacillus subtilis as indicated by its presence in the same node with Bacillus subtilis subsp. spizizenii. The isolate obtained shows good cellulase activity but requires further characterization to determine its potentiality as an industrial producer of cellulase.en_US
dc.description.statementofresponsibilityFaria Mahjabeen
dc.format.extent79 pages
dc.language.isoenen_US
dc.publisherBRAC Universityen_US
dc.rightsBRAC University thesis are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission.
dc.subjectCellulolytic bacteriaen_US
dc.subject16S rRNAen_US
dc.subjectGene sequencingen_US
dc.titleIsolation of cellulolytic bacteria from soil and identification by 16S rRNA gene sequencingen_US
dc.typeThesisen_US
dc.contributor.departmentDepartment of Mathematics and Natural Sciences, BRAC University
dc.description.degreeB. Microbiology


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