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    •   BracU IR
    • School of Data and Sciences (SDS)
    • Department of Mathematics and Natural Sciences (MNS)
    • Bachelor of Science in Microbiology
    • Thesis (Bachelor of Science in Microbiology)
    • View Item
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    Isolation of shiga toxin producing Escherichia coli O157:H7 from Bovine samples of Dhaka, Bangladesh

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    11126008 Micro bio.pdf (16.75Mb)
    Date
    2015-04
    Publisher
    BRAC University
    Author
    Proma, Rhedia Tehrin
    Metadata
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    URI
    http://hdl.handle.net/10361/4160
    Abstract
    Shiga toxin producing Escherichia coli (STEC) have recently emerged as important food-borne pathogens especially serotype O157:H7. Human diseases ranging from mild diarrhea to hemorrhagic colitis, hemolytic uremic syndrome (HUS) and thrombotic thrombocytopenic purpura can be caused by STEC, typically affecting children, elderly and immune-compromised patients. Bangladesh is considered as an endemic area for shiga toxin producing E.coli O157:H7. The study is conducted to isolate E.coli from bovine samples followed by genotyping identification using PCR. For this purpose bovine feces were collected around Dhaka city to isolate E. coli. The samples were first enriched in enrichment broth and then plated onto MacConkey agar. A total of 61 isolates from 7 samples were presumptively selected as E. coli from primary MacConkey plate. The isolates were subjected to detailed biochemical characterizations using Eosin Methylene Blue (EMB) agar medium, Indole production test, Methyl-red test, Voges-Proskauer’s test, Citrate utilization test, Triple Sugar Iron test and fermentation test. Out of 35 samples analyzed, only 22 isolates, gave identical biochemical properties compared to a reference E. coli strain. Culturally and biochemically positive isolates were tested for stx1 and stx2 genes. From all these isolates, no stx1 gene was detected but 3 were detected for stx2. Therefore, this data showed the prevalence of E. coli in Bangladesh and demands for further study for the prevention of diseases.
    Keywords
    Microbiology
    Description
    This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2015.
     
    Cataloged from PDF version of thesis report.
     
    Includes bibliographical references (page 47-48).
    Department
    Department of Mathematical and Natural Science, BRAC University
    Collections
    • Thesis (Bachelor of Science in Microbiology)

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