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    •   BracU IR
    • School of Data and Sciences (SDS)
    • Department of Mathematics and Natural Sciences (MNS)
    • Bachelor of Science in Biotechnology
    • Thesis (Bachelor of Science in Biotechnology)
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    In vitro culture establishment from Cotyledon and Embryonic Axis Explants of two Bangladeshi Sunflower (Helianthus annus L.) varieties

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    In vitro Culture Establishment from Cotyledon and Embryonic .pdf (2.481Mb)
    Date
    2015-03
    Publisher
    BRAC University
    Author
    Chowdhury, Maisha Mosharrat
    Metadata
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    URI
    http://hdl.handle.net/10361/4129
    Abstract
    Sunflower is considered as source of cholesterol free edible oil. Because of cross pollinating nature of this plant, it is difficult to identify true breeding homozygous line, thus, breeding becomes intricate. Besides, due to biotic and abiotic stresses, development of this crop is urgent. This study was aimed to develop a potential alternative to improve qualitative and quantitative production of this crop through basic tissue culture regeneration. Several attempts with various explants have already discovered in past but no initiative was reported for Bangladeshi sunflower varieties. In present study, optimum seed sterilization method; the efficiency of different explants; effect of different growth hormones in different combinations and concentrations and rooting efficiency of initiated shoots were demonstrated for two farmer popular Bangladeshi sunflower varieties, namely BARI Surjomukhi 2 and BRAC Hysun 33. The seeds were washed with 70% ethanol for 3 minutes followed by vigorous hand shaking with 14% Clorox for 20 minutes. To collect explant, named embryonic axis, testas were removed and 3 mm piece from proximal portion of the seed was excised and inoculated in different combinations and concentrations of hormone supplemented media. For explant cotyledon, sterilized seeds were inoculated in germination media to obtain different ages seedlings, thus five, seven and nine days cotyledon explant was collected. In case of explant embryonic axis, BRAC Hysun 33 variety showed maximum (58.33%) regeneration in 1.0 mg/l BAP with 0.1 mg/l NAA hormone supplemented media while in BARI Surjomukhi 2 variety, 2.0 mg/l BAP gave highest (46.67%) regeneration. Elongated shoots were inoculated in 0.2 mg/l IBA containing media for root induction but further studies are needed to optimize rooting media concentration. The second explant, cotyledon remained nonregenerative in both varieties. Further studies need to be done to develop regeneration system from this explant. In future reproducibility of this protocol using embryonic axis needs to be evaluated.
    Keywords
    Biotechnology
    Description
    This thesis report is submitted in partial fulfillment of the requirement for the degree of Bachelor of Science in Biotechnology, 2015.
     
    Cataloged from PDF version of thesis.
     
    Includes bibliographical references (page 48 - 51).
    Department
    Department of Mathematical and Natural Science, BRAC University
    Collections
    • Thesis (Bachelor of Science in Biotechnology)

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