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    •   BracU IR
    • Department of Mathematics and Natural Sciences (MNS)
    • Master of Science in Biotechnology
    • Thesis (Master of Science in Biotechnology)
    • View Item
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    Cloning of an antiporter gene (NHX1) from PCR amplicons into a recombination competent vector containing a constitutive promoter (caMV35S)

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    10176007.pdf (22.65Mb)
    Date
    2011-10
    Publisher
    BRAC University
    Author
    Razzaque, Samsad
    Metadata
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    URI
    http://hdl.handle.net/10361/1665
    Abstract
    The costal belt of Bangladesh is under the threat of increasing salinity and thus reducing plant productivity. Consequently, salinity has become a major concern for ensuring food security. Salt tolerance is known to be a complex trait both genetically as well as physiologically and conferring this tolerance by introducing a single gene is therefore difficult. However, over expression of the vacuolar antiporter gene, NHX1 has been reported to provide salinity tolerance to a good extent. This work was carried out to clone the coding sequence of the rice antiporter gene (applying Gateway Technology) in a binary vector (pH7WG2.0) from where this can be easily introduced into a plant genome with a highly efficient constitutive promoter (CaMV35S). In this study, NHX 1 gene was first cloned into pENTR and confirmed the insertion through PCR, Restriction Digestion and Sequencing technique. Then the gene of interest was recombined from pENTR to the Destination vector (pH7WG2.0) and recombination was confirmed by PCR and Restriction Digestion respectively. Finally, recombinant vector was then transformed into Agrobacterium (the transformation was also confirmed by PCR and Restriction Digestion) to perform Agrobacterium mediated transformation into tomato plant.
    Keywords
    Biotechnology
    Description
    This thesis report is submitted in partial fulfillment of the requirement for the degree of Masters of Science in Biotechnology, 2011
     
    Cataloged from PDF version of thesis report.
     
    Includes bibliographical references (page 84-87).
    Department
    Department of Mathematical and Natural Science, BRAC University
    Collections
    • Thesis (Master of Science in Biotechnology)

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