• Login
    • Library Home
    View Item 
    •   BracU IR
    • Department of Mathematics and Natural Sciences (MNS)
    • Master of Science in Biotechnology
    • Thesis (Master of Science in Biotechnology)
    • View Item
    •   BracU IR
    • Department of Mathematics and Natural Sciences (MNS)
    • Master of Science in Biotechnology
    • Thesis (Master of Science in Biotechnology)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Evaluation of the antioxidant and in vivo anticancer activity of Excoecaria agallocha bark extract

    Thumbnail
    View/Open
    18176002_MNS.pdf (1.851Mb)
    Date
    2019-10
    Publisher
    Brac University
    Author
    Sultana, Sharmin
    Metadata
    Show full item record
    URI
    http://hdl.handle.net/10361/13907
    Abstract
    Mangrove ecosystems have been known to possess diverse secondary metabolites and a good number of bioactive compounds of pharmaceutical importance have been reported from the mangrove plants. Mangrove plants are biochemically unique, producing wide array of natural products with unique bioactivity. This study was undertaken to evaluate the in vitro antioxidant and in vivo anticancer activity of Excoecaria agallocha (bark) which is a mangrove plant. E. agallocha possess various therapeutically important properties and their different parts have been experimented for antioxidant and anticancer activities in various studies. The objective of this study was to evaluate the bark extract of E. agallocha and to find their antioxidant content and also in vivo anticanceractivity in mice model. For this purpose, dried powdered bark of E. agallocha was extracted by kupchan method of solvent-solvent partitioning and after that polarity band was observed by Thin layer of Chromatography (TLC). In thein vitro antioxidant activity test, the extracts were tested for CUPRAC reducing capacity, total antioxidant capacity, total phenolic content and total flavonoid content. Where, Methanolic bark extract showed highest antioxidant content than other solvent extracts. After that anticancer activity was done in mice model, where comparison of the body weight among the groups of mice and hematological parameters was measured by analyzing clinical tests. In sub-chronic toxicity study, methanolic bark extracts of E. agallocha did not produce any significant changes in body weight of mice model when compared to both control groups (negative and positive) of mice. Moreover, there was significant rising of serum SGPT level during the treatment with the plant extract at a drug 400 mg/kg, but no significant changes were observed with 200 mg/kg dose, indicating no acute or sub-chronic toxicity of the extract. In conclusion, E. agallocha bark extract can be considered as a potential anticancer compound which should be validated from other animal model trials. Further studies are required to fractionate the extract, to identify the bioactive compounds, and which may be directed to carry out in vivo studies of its medicinal active components.
    Keywords
    Kupchan method; Antioxidant activity; Anticancer activity; Hemotological parameters
     
    LC Subject Headings
    Thin layer chromatography
     
    Description
    This thesis report is submitted in partial fulfilment of the requirement for the degree of Master of Science in Biotechnology, 2019.
     
    Catalogued from PDF version of thesis.
     
    Includes bibliographical references (pages 118-130).
    Department
    Department of Mathematics and Natural Sciences, Brac University
    Collections
    • Thesis (Master of Science in Biotechnology)

    Copyright © 2008-2019 Ayesha Abed Library, Brac University 
    Contact Us | Send Feedback
     

     

    Policy Guidelines

    • BracU Policy
    • Publisher Policy

    Browse

    All of BracU Institutional RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Copyright © 2008-2019 Ayesha Abed Library, Brac University 
    Contact Us | Send Feedback