BRAC University Institutional Repository

Molecular detection of anticancer protein Azurin in native Pseudomonas isolates

Show simple item record

dc.contributor.advisor Jahan, Nazneen
dc.contributor.author Sarwar, Fahim
dc.date.accessioned 2019-01-27T08:49:17Z
dc.date.available 2019-01-27T08:49:17Z
dc.date.copyright 2018
dc.date.issued 2018
dc.identifier.other ID 13136005
dc.identifier.uri http://hdl.handle.net/10361/11313
dc.description This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Biotechnology, 2018. en_US
dc.description Cataloged from PDF version of thesis.
dc.description Includes bibliographical references (page 31-36).
dc.description.abstract Azurin is a potent anticancer protein which has the ability to interfere in tumor growth. The purpose of this study was to isolate azurin producing native Pseudomonas isolates along with the confirmation of azurin presence using molecular techniques. In this study, azurin presence was Azurin is a potent anticancer protein which has the ability to interfere in tumor growth. The purpose of this study was to isolate azurin producing native Pseudomonas spp along with the confirmation of azurin presence using molecular techniques. Primarily, the isolates were characterized morphologically and identified biochemically as Pseudomonas spp. Presence of azurin gene was tested for 25 environmental (soil) isolates using specific oligonucleotide primers (AZU-F and AZU-R) in a gradient PCR. The extracted total cellular protein was precipitated by 60% ammonium sulfate followed by dialysis and partially purified azurin using SDS-PAGE. Out of 25 isolates, 10 showed the presence of azurin gene by amplifying a single 545 bp DNA fragment in PCR. Total cellular partially purified protein extracted from 10 azurin producing isolates selected from PCR fractionated by SDS PAGE produced patterns with a wide range of discrete bands with the inclusion of 14 kDa Molecular weight. Presence of azurin gene was confirmed by PCR, and partially purified azurin protein was detected by SDS-PAGE according to specific molecular weight. Detection of high yield azurin producing microbial strains can lead us to the development of anticancer drugs. en_US
dc.description.statementofresponsibility Fahim Sarwar
dc.format.extent 41 pages
dc.language.iso en en_US
dc.publisher BRAC University en_US
dc.rights BRAC University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission.
dc.subject Azurin en_US
dc.subject PCR en_US
dc.subject Anticancer protein en_US
dc.subject SDS-PAGE en_US
dc.subject.lcsh Pseudomonas--Molecular aspects.
dc.subject.lcsh Pseudomonas--Biotechnology.
dc.title Molecular detection of anticancer protein Azurin in native Pseudomonas isolates en_US
dc.type Thesis en_US
dc.contributor.department Department of Mathematics and Natural Sciences, BRAC University
dc.description.degree B. Biotechnology


Files in this item

This item appears in the following Collection(s)

Show simple item record

Policy Guidelines

Search BRACU Repository


Advanced Search

Browse

My Account

Statistics