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Determination of the prevalence and virulence properties of aerobic endospore-forming bacteria isolated from commercial packaged foods in Dhaka city

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dc.contributor.advisor Jahan, Nazneen
dc.contributor.author Syeed, Shabnam
dc.date.accessioned 2018-11-08T08:37:49Z
dc.date.available 2018-11-08T08:37:49Z
dc.date.copyright 2018
dc.date.issued 2018-07
dc.identifier.other ID 13236009
dc.identifier.uri http://hdl.handle.net/10361/10829
dc.description This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Biotechnology, 2018. en_US
dc.description Cataloged from PDF version of thesis.
dc.description Includes bibliographical references (page 41-44).
dc.description.abstract Endospore forming bacteria in packaged foods can lead to gastrointestinal disease and food spoilage upon germination in the GI tract or favourable storage conditions. In the current study,14 samples of packaged foods from 4 categories – UHT milk, milk powder, processed honey and fruit juice – were sampled for isolation of aerobic endospore forming bacteria, namely Bacillus spp., with a focus on theBacillus cereus group. A total of 77 isolates were found; 24 isolates were presumptively identified as B. cereus group, amongst which 4 isolates were classified as B. thuringiensis and 2 isolates were presumed to be B.anthracis. The remaining 52 were classified as B. spp. It was found that UHT milk had the lowest prevalence of endospore forming bacteria, while milk powder had the highest. All isolates were tested for production of hydrolytic exoenzymes that hydrolyze starch, casein, lipid and gelatin, while antimicrobial susceptibility of the 24 B. cereus isolates were tested against 25 antibiotics using disk diffusion method. B. cereus isolates were also screened for production of HBL toxin via discontinuous haemolysis on HBL agar. 15 out of 24 isolates showed production of HBL toxin in vitro, showing potential to cause diarrheal syndrome. en_US
dc.description.statementofresponsibility Shabnam Syeed
dc.format.extent 50 pages
dc.language.iso en en_US
dc.publisher BRAC University en_US
dc.rights BRAC University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission.
dc.subject Food packaging en_US
dc.subject Bacillus en_US
dc.subject B. thuringiensis en_US
dc.subject Haemolysin BL en_US
dc.subject.lcsh Food--Packaging.
dc.subject.lcsh Soil microbiology.
dc.title Determination of the prevalence and virulence properties of aerobic endospore-forming bacteria isolated from commercial packaged foods in Dhaka city en_US
dc.type Thesis en_US
dc.contributor.department Department of Mathematics and Natural Sciences, BRAC University
dc.description.degree B. Biotechnology


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