Determination of antimicrobial activity of some commercial fruit (apple, papaya, lemon and strawberry) extracts against bacteria causing urinary tract infection
AuthorLiya, Sabiha Jahan
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Urinary Tract Infection (UTI) is a worldwide phenomenon in modern times which is increasing the dependency on antibiotics for its treatment. The current study was conducted in order to find alternatives to antibiotics by investigating some commercial fruits for their antimicrobial activity. The fruits in this study includes Green Apple (Malus domestica), Papaya (Carica papaya), Lemon (Citrus limon) and Strawberry (Fragaria ananassa) which were used to prepare methanolic and ethanolic extracts through soxhlet extraction technique. The extracts were used against bacteria that causes UTI and five different strains were selected: E. coli ATCC: 15922, E. coli ATCC: 25922, Pseudomonas aeruginosa ATCC: 27853, Enterococcus faecalis ATCC: 29212 and Klebsiella pneumoniae. The fruits were bought from the local market, cut into small slices and were subjected to sun drying to reduce their moisture content. The dried fruit slices were grounded to fine powder and then run through soxhlet extractor to obtain methanolic and ethanolic extracts by using respective solvents. Antimicrobial test of the extracts were conducted by following agar well diffusion method, where ciprofloxacin was used as a positive control and distilled water was used as a negative control. Among the fruits, Apple and Papaya did not show any zone of inhibition against any of the tested bacteria. But both Lemon and Strawberry showed inhibition zone against all of the mentioned bacteria. The ethanolic extracts of Lemon and Strawberry were more potent than the methanolic extracts. Lemon ethanolic extract showed the highest zone of inhibition against Pseudomonas aeruginosa ATCC: 27853 (18.34 ± 0.58) and lowest against Klebsiella pneumoniae (16.00 ± 1.00). Strawberry ethanolic extracts were also more potent than their methanolic extracts. The highest zone of inhibition was seen against Pseudomonas aeruginosa ATCC: 27853 (16.33 ± 0.58) and the lowest one against Klebsiella pneumoniae (13.33 ± 0.58). The results of Lemon and Strawberry can be considered to be used as an antimicrobial agent in treating UTI.