• Login
    • Library Home
    View Item 
    •   BracU IR
    • Department of Mathematics and Natural Sciences (MNS)
    • Bachelor of Science in Microbiology
    • Thesis (Bachelor of Science in Microbiology)
    • View Item
    •   BracU IR
    • Department of Mathematics and Natural Sciences (MNS)
    • Bachelor of Science in Microbiology
    • Thesis (Bachelor of Science in Microbiology)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    In silico B-cell and T-cell epitope-based vaccine designing against Chikungunya virus

    Thumbnail
    View/Open
    14136002_MNS.pdf (864.7Kb)
    Date
    2018-05
    Publisher
    BRAC Univeristy
    Author
    Anushe, Sheikh
    Metadata
    Show full item record
    URI
    http://hdl.handle.net/10361/10292
    Abstract
    Chikungunya virus (CHIKV) is an arthropod-borne alphavirus, belonging to the Togavirus family. There is no commercial treatment or vaccine against CHIKV, despite the acute epidemics taking place in several events distributed among wide areas. In this study, we employed various computational methods to identify B-cell and T-cell epitopes from the envelope protein E1, which have the potential for vaccine development against CHIKV. By analyzing the immune parameters of the conserved sequences of E1 glycoprotein using various databases and bioinformatics tools, we identified one potential B-cell and another T-cell epitope which may be used as epitope-based peptide vaccines. Using two different B-cell epitope prediction servers, five highly similar B cell epitopes were identified from the E1 protein. Immunoinformatics analyses revealed that NTQLSEAHVEKS is a highly conserved, antigenic, surface accessible, flexible and hydrophilic B-cell epitope. Two highly conserved, non- allergenic, non-cytotoxic putative T-cell epitopes having high world population coverages were analyzed for their binding with the HLA-C 12*03 molecule. Docking simulation assay revealed that SASAKLRVL has significantly lower binding energy, which strengthened its potential as being a T-cell epitope for the epitope-based vaccine against CHIKV. This study needs more in vivo investigation. However, mindful of the stability and reproducibility of the immune system at choosing and acting against peptide epitopes, this study allows us to claim a B-cell and a T-cell epitope for the epitope-based peptide vaccine against the E1 protein of CHIKV with good confidence.
    Keywords
    Chikungunya; B-cell; T-cell; Chikungunya vaccine; CHIKV
     
    Description
    This thesis report is submitted in partial fulfilment of the requirement for the degree of B.Sc in Microbiology, 2018.
     
    Catalogued from PDF version of thesis report.
     
    Includes bibliographical references (pages 41-47).
    Department
    Department of Mathematics and Natural Sciences, BRAC University
    Collections
    • Thesis (Bachelor of Science in Microbiology)

    Copyright © 2008-2019 Ayesha Abed Library, Brac University 
    Contact Us | Send Feedback
     

     

    Policy Guidelines

    • BracU Policy
    • Publisher Policy

    Browse

    All of BracU Institutional RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Copyright © 2008-2019 Ayesha Abed Library, Brac University 
    Contact Us | Send Feedback