A study on aluminum toxicity with female rats: can vitamin E help to reduce its effect on biological systems?

Abstract

Increasing complaints about metal toxicity have been reported with the modernization of medical science. However, in this field, aluminum has been a renewed matter of concern. Therefore, more impotence is given to find out natural as well as synthetic substances that can alleviate the toxic effect of aluminum in the body. That is why; this study has aimed to identify the effect of vitamin E in reducing the toxic effect on biological systems caused by Aluminum. The study was conducted for 28 days and with total 18 female albino Wistar rats weighing 138-150g. 18 rats were divided into three groups-control, aluminum treated and aluminum plus vitamin E treated. The control group was receiving normal drinking water along with two drops of Tween 80 whereas aluminum treated group was getting aluminum nitrate solution 1ml/rat containing half ‘x’ of body weight and two drops of Tween 80. Moreover, aluminum plus vitamin E treated group was receiving aluminum nitrate solution 1ml/rat containing half ‘x’ of body weight along with 1ml/rat vitamin containing 22.5 mg of vitamin E per kg and also two drops of Tween 80. After 28 days, the rats were sacrificed, and blood was collected from the heart using ventricular puncture method. The collected blood was sent for hematological and clinical bio-chemistry examinations. At the same time, the liver, skin, brain, kidney and ovary of the rats were collected in the formalin and sent for histopathological study. The clinical biochemistry examination showed significant results for all the parameters except basophils, while the histopathology showed that vitamin E has the potential to eliminate the toxic effects caused by aluminum.