Investigation of in vitro antioxidant and cyto-toxic activity of different fractions of root ex-tracts of Pandanus fascicularis

Abstract

The plant P. fascicularis has significant therapeutic activities in the different parts including bark, stem, leaves, trunk, seed, fruit and flower. Every part shows unique medicinal activities. They exert medicinal efficiency that can be incorporated to treat diverse ailments and can develop new formulas essential to treat newer afflictions. But the root did not show any significance yet so we are trying to emphasize on the antioxidant and cytotoxic activity of the roots. Ethanol (EPF), ethyl acetate (EAPF) and chloroform extract (CLPF) of the grinded root were prepared for running the experiments. The anti-oxidant study was observed in vitro by DPPH scavenging radical in com-parison to ascorbic acid as standard at 517 nm. The IC50 value of standard Ascorbic acid EAPF, EPF and CLPF extracts were 56.27, 46.35, 35.5, 48.25 and 50.39, respectively. From the obtained results of the extracts are considered to have antioxidant effect and CHPF was found to be higher than the other extracts. And in the case of brine shrimp lethality bioassay the EPF showed higher value of LC50 compared with the other two extracts. Here vincristine sulphate which was used as positive control. It was observed that the EAPF gave higher value of LC50. It means that using small amount of EPF will give more toxic effect. The LC50 values of the vincristine sulphate, EAPF, EPF and CLPF extracts were 6.73, 8.09, 9.015, and 7.046, respectively. So it can be claimed that the results obtained from the experimental data were satisfactory enough to run further studies considering different parameters such as solvent type- methanol, acetone, pet ether etc. We can conclude that the root extract of P. fascicularis contains active antioxidant and cytotoxic functions with great potential for producing a revolutionary drug.