Phytochemical screening and study of analgesic potential of Pandanus fascicularis different root extract in experimental animal model

Abstract

The aim of this study was to investigate phytochemicalcompoundsand study of analgesic activity ofdifferent root extracts like [ethanol (EAPF), chloroform (CHPF) and ethyl acetate (EAPF)]ofPandanusfascicularis. For analgesic activity we have performed acetic acid induced writhing method and formalin induced pain method. Different extracts were tested for qualitative chemical analysis which reveals the presence of alkaloids, glycosides, carbohydrate and tannin. In our present study we administered our experimented crude extracts at two doses 250 mg/kg and 500 mg/kg to evaluate the analgesic activity in rodents. Both doses of extracts significantly (p<0.01) inhibited writhing response induced by acetic acid and formalin. The experimental extracts have satisfactory analgesic effect compare to control group and represented by number of writhes in acetic acid induced pain method as well as number of licking time in formalin induced licking method. The EAPF extract, at 250 mg/kg dose showed 85.7 % and at 500 mg/kg showed 88.12 % inhibition of writing response compare to reference Indomethacine(58.50%) inhibition was observed. In conclusion we can say that the different root extracts of Pandanusfascicularis contain some active phytochemical constituents which are responsible for the observation of analgesic activity and this amply justify the traditional use of this plant as a pain reliever.