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Investigation of phytochemical and in-vitro biological potential of anisoptera scaphula

2016-10-01T00:00:00Z

Title: Investigation of phytochemical and in-vitro biological potential of anisoptera scaphula Authors: Naher, Airin Abstract: This study was intended to identify and assess the potential phytochemical as well as biological properties of the medicinal plant Anisoptrea scaphula (family: Dipterocarpaceae). To fulfill these purposes, different experiments were carried out, such as phytochemical screening, antioxidant activity test, brine shrimp lethality bioassay and thrombolytic activity test. It is evident from different observations that, this plant is an abundant source of large number of long chain hydrocarbons, beta-setosterol, beta-setosterolpalmitate, beta-setosterol-beta-D-glucosidase, mannitol. In the existing investigation, phytochemical screening has indicated the presence of tannins, saponins, carbohydrates, glycosides and glucosides in this particular plant. Additionally, moderate antioxidant, antimicrobial activity and thrombolytic activity ofAnisoptrea scaphula were observed from the experiments. However, it showed significant cytotoxicityin brine shrimp lethality bioassay. So, on the basis of the present investigation, it can be proposed that the plant “Anisoptrea scaphula” can be used as a medicinal plant. Description: This project report is submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2016; Cataloged from PDF version of project report.; Includes bibliographical references (page 61-63).

In vitro biological investigation of eichhornia crassipes (pontederiaceae)

2016-11-17T00:00:00Z

Title: In vitro biological investigation of eichhornia crassipes (pontederiaceae) Authors: Islam, Farjana Abstract: Eichhornia crassipesis free floating aquatic plant and it belongs to the family Potenderiaecae and it is very native to South America. Though it is an aquatic plant but it also has therapeutic effect. Extracts of the various parts of the E. crassipes has therapeutic effect such as antimicrobial activity, anti-oxidant, wound healing, antitumor, cytotoxic, larvicidal activity. Leaf extract of this plat contain phenolic compounds, alkaloids, terpenoids, glycosides, sterols. The objective of this study was to investigate the bioactivity like cytotoxicity by lethal brine shrimp method, antimicrobial activity by disc diffusion methods from all the extractive of Eichhornia crassipes. The lethal brine shrimp study reveals the mild to moderate result by LC50. In antimicrobial test, this pant has negligible potentiality to act against bacterial and fungal strain. Description: This project report is submitted in a partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2016; Cataloged from PDF version of project report.; Includes bibliographical references (page 34-36).

DoE based formulation and optimization of sustained release tablet dosage form of diacerein

2016-02-01T00:00:00Z

Title: DoE based formulation and optimization of sustained release tablet dosage form of diacerein Authors: Syeara, Nausheen Abstract: Osteoarthritis is a type of arthritis that occurs mainly in elderly people and is characterized by pain in joints, stiffness, etc. which may lead to disability. Diacerein is a new anti-inflammatory drug which is more profoundly said to be an anti-arthritic agent since pharmacological studies found it to be effective in all kinds of arthritis as analgesic and anti-inflammatory. Moreover, it is also as agent that brings about structural change unlike normal NSAIDs. The aim of the study was to develop a sustained release tablet dosage form of diacerein that will be an optimized formulation and highly patient compliant. The method involves designing the formulation and controlling release by using blends of two HPMC based release retardants. Drug-excipient compatibility tests were carried out using DSC and FTIR spectroscopy. Based on the fact that no incompatibility was present, step was taken to prepare trial batches of tablets of nine different formulations. The physical evaluation of both the drug-excipient powder mixtures and tablets of the nine formulations were performed and results were found to be within acceptable ranges for all tests. Dissolution profile of tablets were done and the release data was then put into zero order, first order, Higuchi, Korsmeyer-Peppas and Hixon-Crowell kinetic models. The release data was simulated in Design Expert Software to carry out statistical analysis and the effect of both polymers on release patterns were observed after 1,4 and 8 hours in terms of contour plots and 3D surface plots, which were all significant (p<0.05) . Finally, the optimization overlay plot was obtained from which the optimized formulation was determined. Description: This project report is submitted in a partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2016; Cataloged from PDF version of project report.; Includes bibliographical references (page 73-75).

Investigation of in-vitro antioxidant potential in crotalaria verrucosa along with identification and quantification of its polyphenolic compounds

2016-02-01T00:00:00Z

Title: Investigation of in-vitro antioxidant potential in crotalaria verrucosa along with identification and quantification of its polyphenolic compounds Authors: Ahmed, Zainab Syed Abstract: Crotalaria verrucosa (Fabaceae) is a local medicinal plant, native to Chittagong, Khulna, Rajshahi and Sylhet, in Bangladesh. Traditionally, C. verrucosa is used for the treatment of folkloric remedies namely, scabies, heart complaints as well as in the treatment of throat and oral diseases which may be extrapolated to its antioxidant potential as deduced from this study. In this study, the preliminary phytochemical screening of methanolic leaf extract of C. verrucosa showed the presence of flavonoids, phenolic compounds, alkaloids, tannin, steroids and glycosides. Various in-vitro antioxidant studies were performed to determine its antioxidant potential. The extract showed moderate antioxidant activity in DPPH free radical scavenging assay where ascorbic acid was used as the reference standard. Also, using the DPPH free radical scavenging assay, the IC50 values of Crotalaria verrucosa and ascorbic acid was found to be 533.738ug/mL and 154.916ug/mL, respectively. Whilst the total phenolic content was found to be 152.180mg/g of gallic acid equivalent (GAE), the total flavonoid content was found to be much higher, 184.510mg/g of quercetin equivalent (QE). The total antioxidant capacity was determined to be 32.342mg of ascorbic acid equivalent (AAE) for a concentration of 1200ug/mL of methanolic extract of C. verrucosa. In addition to the determination of in vitro antioxidant potential, HPLC coupled with a diode-array-detector was used to identify and quantify the polyphenolic compounds present in the methanolic crude extract of C. verrucosa. Amongst the polyphenolic compounds, the most notable of them were identified as gallic acid, (+)-catechin hydrate, vanillic acid, caffeic acid, syringic acid, (–)-epicatechin and vanillin were identified present in concentrations of 19.53, 5.08, 5.97, 6.22, 1.09, 7.16 and 8.05mg/100g of dried extract. Amongst the polyphenolic compounds quantified, gallic acid was most predominantly present (19.53mg/100g of dried extract). Although vanillic acid is a catechin-metabolite; (+)-catechin hydrate and (–)-epicatechin are flavonoids and gallic acid, syringic acid, caffeic acid and vanillin are polyphenolic compounds, all of these compounds possess established antioxidant activities. Description: This project report is submitted in a partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2016; Cataloged from PDF version of project report.; Includes bibliographical references (page 84-91).